Supported planar bilayer formation by vesicle fusion: the interaction of phospholipid vesicles with surfaces and the effect of gramicidin on bilayer properties using atomic force microscopy
Supported planar bilayer formation by vesicle fusion: the interaction of phospholipid vesicles with surfaces and the effect of gramicidin on bilayer properties using atomic force microscopy
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DOI：
10.1016/s0005-2736(00)00288-1
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发表时间：
2000-12-20
影响因子：
3.4
通讯作者：
Cramb, DT
中科院分区：
文献类型：
--
作者：
Leonenko, ZV;Carnini, A;Cramb, DT
利用磁交流模式原子力显微镜（MAC-AFM）研究了囊泡融合法制备支撑磷脂双分子层（SPB）的过程。所研究的系统是二油酰磷脂酰胆碱（DOPC）的云母和云母与3-氨丙基三乙氧基硅烷（APTES），DOPC囊泡与短杆菌肽纳入云母和APTES改性云母。AFM图像揭示了双层形成的三个阶段：局部盘状特征，即囊泡的单个双层足迹，部分连续覆盖，最后完全双层形成。支持磷脂双层形成的机制是近端囊泡的融合，而不是表面盘迁移。这种机制似乎不受短杆菌肽的掺入或表面改性的影响。一旦形成，双层就形成一个双层深的圆形缺陷。这些缺陷的尺寸和数量不断增长，直到达到动态平衡。(C)2000 Elsevier Science B. V.保留所有权利。
We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES), and DOPC vesicles with gramicidin incorporated on mica and APTES-modified mica. The AFM images reveal three stages of bilayer formation: localized disklike features that are single bilayer footprints of the vesicles, partial continuous coverage, and finally complete bilayer formation. The mechanism of supported phospholipid bilayers formation is the fusion of proximal vesicles, rather than surface disk migration. This mechanism does not appear to be affected by incorporation of gramicidin or by surface modification. Once formed, the bilayer develops circular defects one bilayer deep. These defects grow in size and number until a dynamic equilibrium is reached. (C) 2000 Elsevier Science B.V. All rights reserved.