Novel Genetic and Molecular Tools for the Investigation and Control of Dengue Virus Transmission by Mosquitoes.
Novel Genetic and Molecular Tools for the Investigation and Control of Dengue Virus Transmission by Mosquitoes.
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DOI：
10.1007/s40475-013-0007-2
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发表时间：
2014-03-01
影响因子：
5.4
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中科院分区：
文献类型：
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埃及伊蚊是整个热带世界登革热病毒 (DENV) 的主要传播媒介。这种嗜人性蚊子需要持续感染登革热病毒，然后才能通过唾液将病毒传播给新的脊椎动物宿主。在蚊子中，DENV 面临多种先天免疫途径，其中 RNA 干扰被认为是最重要的。艾埃。埃及伊蚊基因组项目为病原体与媒介相互作用的高级分子研究打开了大门，包括用于基础研究和媒介控制目的的媒介基因操作。因此，艾伊.埃及伊蚊已成为在分子水平上研究虫媒病毒载体能力的主要模型。在这里，我们介绍了有关 DENV-蚊子相互作用的最新发现，强调先天免疫反应如何调节白伊蚊的 DENV 感染。埃及伊蚊。我们还描述了AE基因操作的最新进展。埃及伊蚊并讨论了如何利用该技术在分子水平上研究登革热病毒的媒介传播并控制病毒在现场的传播。
Aedes aegypti is the principal vector of dengue virus (DENV) throughout the tropical world. This anthropophilic mosquito species needs to be persistently infected with DENV before it can transmit the virus through its saliva to a new vertebrate host. In the mosquito, DENV is confronted with several innate immune pathways, among which RNA interference is considered the most important. The Ae. aegypti genome project opened the doors for advanced molecular studies on pathogen-vector interactions including genetic manipulation of the vector for basic research and vector control purposes. Thus, Ae. aegypti has become the primary model for studying vector competence for arboviruses at the molecular level. Here, we present recent findings regarding DENV-mosquito interactions, emphasizing how innate immune responses modulate DENV infections in Ae. aegypti. We also describe the latest advancements in genetic manipulation of Ae. aegypti and discuss how this technology can be used to investigate vector transmission of DENV at the molecular level and to control transmission of the virus in the field.