OVARIAN SYMPATHECTOMY IN THE GUINEA-PIG .1. EFFECTS ON FOLLICULAR DEVELOPMENT DURING THE ESTROUS-CYCLE
OVARIAN SYMPATHECTOMY IN THE GUINEA-PIG .1. EFFECTS ON FOLLICULAR DEVELOPMENT DURING THE ESTROUS-CYCLE
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DOI：
10.1007/bf00214226
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发表时间：
1984-01-01
影响因子：
3.6
通讯作者：
BURDEN, HW
中科院分区：
文献类型：
--
作者：
CURRY, TE;LAWRENCE, IE;BURDEN, HW
在成年豚鼠的动情周期中，卵巢肾上腺素能神经对卵泡生长的影响是通过比较同一动物的对照和化学交感神经切除卵巢中的卵泡发育来确定的。选择性卵巢交感神经切除术是通过在周期的第2天（第1天=发情日）将6-羟基多巴胺注射到手术闭合的卵巢膜囊（囊）中来实现的。对侧手术闭合的卵巢囊注射用于6-羟基多巴胺的溶剂。在周期的第5、10和14天对动物进行剖腹。从双侧子宫卵巢静脉采集血液用于测量孕酮和雄烯二酮。对卵巢进行组织学检查，并对每个卵巢中的卵泡数量进行形态计量学分析。第2天的交感神经切除导致周期第10天健康的排卵前卵泡（直径> 700 μ m）减少。此时卵巢重量或每个卵巢的卵泡总数无差异。在周期的第5天和第14天，卵巢重量、每个卵巢的卵泡总数或任何大小分类的卵泡均无差异。与对侧对照水平相比，交感神经切除术并没有改变子宫卵巢静脉中的孕酮水平。从控制卵巢，有一个显着增加孕酮在血液中的子宫卵巢静脉的第10天，但静脉水平的孕酮交感神经切除卵巢没有显着不同的任何一天的周期。在交感神经切除卵巢的静脉流出物中，雄烯二酮在第5天比第10天和第14天升高。卵巢肾上腺素能神经可能调节大窦卵泡的生长和卵巢激素的生成。
The influence of ovarian adrenergic nerves on follicular growth during the estrous cycle in the adult guinea pig was ascertained by comparing follicular development in control and chemically sympathectomized ovaries from the same animal. Selective ovarian sympathectomy was achieved by injecting 6-hydroxydopamine into a surgically closed periovarian membranous sac (bursa) on day 2 of the cycle (day 1 = day of estrus). The contralateral surgically closed ovarian bursa was injected with solvent used for 6-hydroxydopamine. Animals were laparotomized on days 5, 10 and 14 of the cycle. Blood from the utero-ovarian vein was collected bilaterally for measurement of progesterone and androstenedione. The ovaries were processed for histologic examination, and the number of follicles in each ovary was analyzed morphometrically. Sympathectomy on day 2 caused a decrease in healthy preovulatory follicles (> 700 .mu.m diameter) on day 10 of the cycle. There were no differences in ovarian weights or the total number of follicles per ovary at this time. On days 5 and 14 of the cycle, there were no differences in ovarian weights, total number of follicles per ovary or follicles in any size classification. Sympathectomy did not alter progesterone levels in the utero- ovarian vein as compared to contralateral control levels. From control ovaries, there was a significant increase in progesterone in the blood of the utero-ovarian vein on day 10 but venous levels of progesterone from sympathectomized ovaries were not significantly different at any day of the cycle. In the venous effluent from sympathectomized ovaries, androstenedione was elevated at day 5 compared to days 10 and 14. Ovarian adrenergic nerves may modulate growth of large antral follicles and ovarian steroidogenesis during the estrous cycle.