Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus
Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus
复制标题
体腔液的代谢组学分析揭示了海参刺参取内脏行为的生理机制
DOI：
10.1016/j.aquaculture.2021.736960
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发表时间：
2021-05-31
期刊：
影响因子：
4.5
通讯作者：
Zhang, Shuangli
中科院分区：
文献类型：
--
作者：
Ding, Kui;Zhang, Libin;Zhang, Shuangli
除内脏是海参在养殖和运输过程中经常发生的一种特殊行为。这种行为涉及一个复杂的生理过程，并导致消化道和其他脏器的排出。然而，对海参内脏的研究尚不充分，因此确定海参内脏的内部调节代谢物和潜在途径可能为这一特定行为提供科学依据。本研究采用超高效液相色谱-四极杆飞行时间质谱法（ulc - q - tof - ms）对日本刺参内脏排出的体腔液和正常日本刺参体腔内排出的体腔液进行分析，检测其内脏行为过程中代谢物和代谢途径的变化。总的来说，在海参内脏排出的体腔液体中，发现有9种代谢物增加，11种代谢物减少。这些代谢物包括磷脂酰乙醇胺、葡萄糖神经酰胺、l -色氨酸、氨基甲酸和环己胺。此外，代谢途径的富集分析还揭示了5条显著改变的信号通路：糖基磷脂酰肌醇（GPI）锚定生物合成、自噬调节、鞘脂代谢、氮代谢以及苯丙氨酸、酪氨酸和色氨酸的生物合成。这些结果为日本刺参内脏行为的潜在生理机制提供了有价值的数据，对海参的养殖和运输具有重要意义。
Evisceration is a peculiar behavior that happens frequently in the processes of aquaculture and transportation of sea cucumbers. This behavior involves a complex physiological process and results in the expulsion of the digestive tract and other viscera. However, studies on evisceration are insufficient, and the identification of the internal regulatory metabolites and potential pathways of evisceration in the sea cucumber are therefore likely to provide a scientific basis for this specific behavior. In this study, ultraperformance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was performed on the coelomic fluids that ejected by evisceration Apostichopus japonicus and in the coeloms of normal A. japonicus to detect changes in metabolites and metabolic pathways in the process of evisceration behavior. In total, nine metabolites were found to have increased and eleven to have decreased in the ejected coelomic fluids of evisceration sea cucumbers. These metabolites included phosphatidylethanolamine, glucosylceramide, L-tryptophan, carbamic acid, and cyclohexylamine. In addition, enrichment of metabolic pathway analyses revealed five significantly changed signaling pathways: Glycosyl-phosphatidyl-inositol (GPI)-anchor biosynthesis, regulation of autophagy, sphingolipid metabolism, nitrogen metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis. These results contribute valuable data on the potential physiological mechanisms underlying evisceration behavior of A. japonicus, which could have important implications for the aquaculture and transportation of sea cucumbers.