Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes
Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes
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DOI：
10.1091/mbc.8.11.2119
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发表时间：
1997-11-01
影响因子：
3.3
通讯作者：
Mastronarde, DN
中科院分区：
文献类型：
--
作者：
Winey, M;Yarar, D;Mastronarde, DN
通过计算机辅助从连续切片细胞的电子显微镜图像重建整个细胞核，确定了酿酒酵母单个细胞核中的核孔复合体(NPC)数量。对细胞周期中不同时间点的32个单倍体细胞的细胞核进行了建模，发现其含有65到182个核祖细胞。形态标记，如细胞形状和核形状，被用来确定被检查细胞的细胞周期阶段。鼻咽癌数量与细胞周期阶段相关，表明鼻咽癌细胞数量从G(1)期开始稳步增加，提示鼻咽癌组装在整个细胞周期中持续发生。然而，在细胞周期中观察到的由核表面积表示的核被膜的积累并不是以相同的速度连续的，以至于单位面积核被膜上的神经前体细胞密度在明显的S期细胞中达到峰值。对核包膜重建的分析也表明，鼻咽癌与鼻咽癌之间的距离并不理想。然而，在核膜区域的大簇中发现了NPC。有趣的是，在早期有丝分裂核中，NPC簇最明显，并被发现与纺锤体极体有关，但这种联系的功能意义尚不清楚。
The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of serially sectioned cells. Nuclei of 32 haploid cells at various points in the cell cycle were modeled and found to contain between 65 and 182 NPCs. Morphological markers, such as cell shape and nuclear shape, were used to determine the cell cycle stage of the cell being examined. NPC number was correlated with cell cycle stage to reveal that the number of NPCs increases steadily, beginning in G(1)-phase, suggesting that NPC assembly occurs continuously throughout the cell cycle. However, the accumulation of nuclear envelope observed during the cell cycle, indicated by nuclear surface area, is not continuous at the same rate, such that the density of NPCs per unit area of nuclear envelope peaks in apparent S-phase cells. Analysis of the nuclear envelope reconstructions also revealed no preferred NPC-to-NPC distance. However, NPCs were found in large clusters over regions of the nuclear envelope. Interestingly, clusters of NPCs were most pronounced in early mitotic nuclei and were found to be associated with the spindle pole bodies, but the functional significance of this association is unknown.