Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris
Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris
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DOI：
10.1093/pcp/pcd001
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发表时间：
2000-07-01
影响因子：
4.9
通讯作者：
Hashimoto, T
中科院分区：
文献类型：
--
作者：
Shoji, T;Yamada, Y;Hashimoto, T
尼古丁生物碱是在烟草属植物的根中合成的，经过虫害、伤害和茉莉酸处理后，其合成增加。腐胺N-甲基转移酶(PMT)催化尼古丁生物合成的第一步，本文报道了三个烟草PMT基因(NsPMT1、NsPMT2和NsPMT3)的表达模式。NsPMT基因的转录本仅在根中检测到，并被茉莉酸甲酯处理上调。当NsPMT1、NsPMT2和NsPMT3的5‘侧翼区分别与β-葡萄糖醛酸酶报告基因融合后，通过农杆菌介导法导入野百合，所有外源基因均在根部的皮层、内皮层和木质部表达，并被茉莉酸甲酯处理上调。NsPMT基因的这些定性相似的表达模式是通过其保守的5‘侧翼区域仅0.25kb实现的，该区域不包含已知的茉莉酸反应元件。
Nicotine alkaloids are synthesized in the root of Nicotiana species, and their synthesis increases after insect attack, wounding and jasmonate treatment of the leaf. Putrescine N-methyltransferase (PMT) catalyzes the first committed step in nicotine biosynthesis, The expression patterns of the three Nicotiana sylvestris PMT genes (NsPMT1, NsPMT2, and NsPMT3) are reported in this study. Transcripts of the NsPMT genes were detected only in the root, and were up-regulated by methyl jasmonate treatment. When the 5'-flanking regions of NsPMT1, NsPMT2, and NsPMT3 were fused independently to beta-glucuronidase reporter gene and introduced into N. sylvestris by Agrobacterium-mediated transformation, all introduced transgenes were expressed in the cortex, endodermis, and xylem in the root, as well as upregulated by methyl jasmonate treatment. These qualitatively similar patterns of expression for the NsPMT genes are achieved with only 0.25 kb of their conserved 5'-flanking regions, which contained no known jasmonate-responsive elements.