Application of mycobacterial interspersed repetitive unit typing to Manitoba tuberculosis cases: Can restriction fragment length polymorphism be forgotten?
Application of mycobacterial interspersed repetitive unit typing to Manitoba tuberculosis cases: Can restriction fragment length polymorphism be forgotten?
复制标题
DOI：
10.1128/jcm.42.11.5001-5006.2004
复制
发表时间：
2004-11-01
影响因子：
9.4
通讯作者：
Kabani, AM
中科院分区：
文献类型：
--
作者：
Blackwood, KS;Wolfe, JN;Kabani, AM
自 1993 年以来，加拿大马尼托巴省回收的所有结核分枝杆菌分离株均采用标准 IS6110 限制性片段长度多态性 (RFLP) 方法进行基因分型，用于常规监测、预防和控制目的。迄今为止，我们的实验室已收集了 1,290 个分离株，并从中鉴定出大约 390 个独特的指纹模式或“类型”。尽管众所周知，标准方法是一种冗长且劳动密集型的程序，但分枝杆菌散布重复单位变量串联重复 (MIRU-VNTR) 方法是结核病分离株分型的更有效替代方法，最近已获得认可。因此，2003 年获得的所有分离株 (n = 126) 都通过这两种方法进行了分型，以确定用 MIRU 分型替代 RFLP 方法对所有未来分离株的效用。将 Hunter 判别指数应用于现有研究人群表明，MIRU 方法的判别力 (D) 与 RFLP 方法接近（D-MIRU = 0.831 至 0.984 对比 D-RFLP = 0.821 至 0.997）。通过使用与 RELP 衍生聚类相关的 MIRU 数据对分离株进行聚类，为调查或确认近期传播的发生率提供有用信息。此外，还确定曼尼托巴省的每种主要 RELP 类型都有相应的、可识别的 MIRU 类型。可以想象，未来 RFLP 分型可以用 MIRU 取代，在全省进行实时、持续的结核病监测。
Since 1993, all Mycobacterium tuberculosis isolates recovered in the province of Manitoba, Canada, have been genotyped by the standard IS6110-restriction fragment length polymorphism (RFLP) method for routine surveillance, prevention, and control purposes. To date, our laboratory has collected 1,290 isolates, from which we have identified approximately 390 unique fingerprint patterns or "types." Although the standard method is well known for being a lengthy and labor-intensive procedure, a more efficient alternative for typing tuberculosis isolates, the mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) method, has recently gained acceptance. Consequently, all isolates acquired in 2003 (n = 126) were typed by both methods in order to determine the utility of replacing the RFLP method with MIRU typing for all future isolates. Application of Hunter's discriminatory index to the available study population showed that the MIRU method was close in discriminatory power (D) to the RFLP method (D-MIRU = 0.831 to 0.984 versus D-RFLP = 0.821 to 0.997). Clustering of isolates by using MIRU data correlated with RELP-derived clustering, lending useful information for either an investigation or confirmation of an incidence of recent transmission. In addition, it was determined that each predominant RELP type in Manitoba had a corresponding, recognizable MIRU type. It is conceivable that in the future RFLP typing can be replaced with MIRU for real-time, ongoing tuberculosis surveillance in the province.