MECHANISM FOR 193-NM LASER RADIATION-INDUCED EFFECTS ON MAMMALIAN-CELLS
MECHANISM FOR 193-NM LASER RADIATION-INDUCED EFFECTS ON MAMMALIAN-CELLS
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DOI：
10.2307/3577598
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发表时间：
1990-05-01
影响因子：
3.4
通讯作者：
MIRRO, J
中科院分区：
文献类型：
--
作者：
KOCHEVAR, IE;WALSH, AA;MIRRO, J
研究了氟化氩准分子激光193 nm辐射对哺乳动物细胞损伤的部位。中国仓鼠卵巢细胞还原四唑染料（MTT）的能力降低到未照射对照的37%，降低了2.5倍。103 J/m2的193-nm辐射时，测量或照射后4或24小时。相比之下，抑制MTT减少254 nm辐射，主要导致DNA损伤是不可测量的，使用该试验在4小时后曝光;在24小时45 J/m2抑制MTT减少到37%的控制。在质膜渗透性的增加，检测51铬释放，观察到在15分钟内暴露于193-nm辐射，而暴露于254-nm辐射并没有导致这种立即释放的51铬。在对照实验中，线粒体毒物，羰基氰间氯苯腙，没有导致51铬释放在黑暗中，表明193纳米辐射诱导的质膜渗透性的增加是不是随后的线粒体功能丧失。使用低193 nm通量从C4 H10 T1/2细胞中释放[3 H]-花生四烯酸，而使用UVB（290-32 nm）辐射释放[3 H]花生四烯酸需要细胞毒性通量。DNA似乎不是193 nm诱导的细胞损伤的主要位点，因为在暴露于冰上的细胞中没有检测到碱不稳定位点，其暴露时间高达2x。104 J/m2的193 nm辐射。这些结果表明，193-nm辐射产生的质膜水平上的主要损害。
The cellular sites for damage in mammalian cells caused by 193-nm radiation from an argon fluoride excimer laser were investigated. The ability of Chinese hamster ovary cells to reduce a tetrazolium dye (MTT) was decreased to 37% of unirradiated control by 2.5 .times. 103 J/m2 of 193-nm radiation when measured either 4 or 24 h after irradiation. In contrast, inhibition of MTT reduction by 254-nm radiation which primarily causes DNA damage was not measurable using this assay at 4 h after exposure; at 24 h 45 J/m2 inhibited MTT reduction to 37% of control. An increase in plasma membrane permeability, detected by 51Cr release, was observed within 15 min of exposure to 193-nm radiation, whereas exposure to 254-nm radiation did not cause this immediate release of 51Cr. In control experiments, the mitochondrial poison, carbonyl cyanide m-chlorophenyl hydrazone, did not cause 51Cr release in the dark, indicating that the 193-nm radiation-induced increase in plasma membrane permeability was not subsequent to loss of mitochondrial function. [3H]-Arachidonic acid was released from C4H10T1/2 cells using low 193-nm fluences, whereas release of [3H]arachidonic acid using UVB (290-32 nm) radiation required cytotoxic fluences. DNA does not appear to be a major site of 193 nm-induced cellular damage because alkali-labile sites were not detected in cells exposed on ice to up to 2 .times. 104 J/m2 of 193-nm radiation. These results indicate that 193-nm radiation produces primary damage on the level of the plasma membrane.