Herpesviruses and human endogenous retroviral sequences in the cerebrospinal fluid of multiple sclerosis patients
Herpesviruses and human endogenous retroviral sequences in the cerebrospinal fluid of multiple sclerosis patients
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DOI：
10.1177/1352458507086425
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发表时间：
2008-06-01
影响因子：
5.8
通讯作者：
Arroyo, R.
中科院分区：
文献类型：
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作者：
Alvarez-Lafuente, R.;Garcia-Montojo, M.;Arroyo, R.
目的探讨人类疱疹病毒(HHV)和人类内源性逆转录病毒(HERV)感染在多发性硬化(MS)发病机制中的作用。方法收集92例脑脊液(CSF)标本，其中48例为首次临床表现为脱髓鞘的MS患者，23例为其他炎症性神经疾病(OIND)患者，21例为其他非炎症性神经疾病(ONIND)患者。结果(1)单纯疱疹病毒、水痘-带状疱疹病毒、EB病毒、巨细胞病毒、HHV-6、HERV-H和HERV-W在MS患者和对照组脑脊液中的感染率和病毒载量分别为1/48(2.1%，86拷贝/ml)和1/23(4.3%，115.2拷贝/ml)；(2)48例MS患者中仅有1例(2.1%，72拷贝/毫升脑脊液)检出EB病毒，(3)48例患者中仅5例(10.4%)脑脊液中检出HHV-6基因(128.1拷贝/毫升脑脊液)，(4)未发现VZV、CMV、HERV-H和HERV-W阳性病例，未发现混合感染病例；(6)MS患者HHV总感染率为7/48(14.6%)，对照组为1/44(2.3%)(P=0.038)。结论MS患者脑脊液中HHV感染的发生率高于其他神经系统疾病患者，但仅HHV-6与MS的发病有关。
Objective To analyze the possible role of human herpesvirus ( HHVs) and human endogenous retroviruses ( HERVs) infection in multiple sclerosis ( MS) pathogenesis.Methods A total of 92 cerebrospinal fluid ( CSF) samples were collected: 48 from MS patients at the first clinically evident demyelinating event, 23 from patients with other inflammatory neurological diseases ( OINDs) and 21 from patients with other non-inflammatory neurological diseases (ONINDs). Total DNA and RNA were isolated, and the prevalences and viral loads of herpes simplex virus ( HSV), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), HHV-6, HERV-H and HERV-W in the CSF of MS patients and controls were evaluated using a quantitative real-time polymerase chain reaction assay.Results (i) For HSV, 1/48 (2.1%, 86 copies/ml of CSF) MS patients and 1/23 (4.3%, 115.2 copies/ml of CSF) OIND patients ( a myelitis case) had HSV sequences in the CSF; ( ii) for EBV, only 1/48 ( 2.1%, 72 copies/ml of CSF) MS patients was positive for EBV; (iii) for HHV-6, only 5/48 (10.4%) MS patients had HHV-6 genomes in their CSF (128.1 copies/ml of CSF); (iv) we did not find any positive cases for VZV, CMV, HERV-H and HERV-W among MS patients or controls; ( v) no cases of co-infections were found; ( vi) the whole prevalence of HHVs was 7/48 (14.6%) for MS patients and 1/44 (2.3%) for controls (p=0.038).Conclusion The findings described here show that HHV infection is more frequent in the CSF of MS patients than in patients with other neurological diseases; however, only HHV-6 seems to be involved in the pathogenesis of MS in a subset of patients.