Organization of angiotensin II immunoreactive cells and fibers in the rat central nervous system. An immunohistochemical study.
Organization of angiotensin II immunoreactive cells and fibers in the rat central nervous system. An immunohistochemical study.
复制标题
大鼠中枢神经系统中血管紧张素 II 免疫反应细胞和纤维的组织。
DOI：
10.1159/000124046
复制
发表时间：
1985
影响因子：
4.1
通讯作者：
Ganten,D
中科院分区：
文献类型：
--
作者：
Lind,RW;Swanson,LW;Ganten,D
血管紧张素II（AII）的免疫反应细胞和纤维的分布进行了检查，在成年雄性SD大鼠与秋水仙碱预处理。与许多其他神经肽的情况一样，AII优先存在于脑干、下丘脑和边缘系统结构中，这些结构参与控制稳态功能。AII染色的细胞体在室旁核和视上核的大细胞部分最突出，在前者的小细胞部分也发现了细胞。其他含有细胞体的下丘脑核包括视交叉上核、内侧视前区和外侧下丘脑区的穹窿周围部分。相当有趣的是，在几个脑室周围器官，特别是穹窿下器官，在那里的细胞和纤维被发现的鲁棒染色。缺水和肾切除术的结果表明，这种染色并不代表循环肽的摄取，而是代表AII的神经连接。在丘脑，AII染色细胞被发现在室旁核，中央内侧核，核reuniens，和喙部的齿状回。两个细胞群在基底端脑，在背侧部的床核的终纹和杏仁核的内侧，躺在任何一个通过终纹染色的通路的两端。在中脑，免疫反应阳性细胞被发现在脚间和脚周核，和一个脑桥细胞群被检测到在外侧臂旁核的最外侧部分。延髓中唯一的AII染色细胞位于孤束核中，靠近最后区的边缘。在中枢神经系统的各个层面都发现了纤维，从嗅球到脊髓，在胶状质和中间外侧细胞柱中观察到终末场。纵向取向的纤维存在于整个室周纤维系统和内侧前脑束，包括其在脑干腹外侧部分的尾侧延伸。研究表明，在许多不同水平上，AII既是体液平衡的激素，又是神经递质。
The distribution of angiotensin II (AII)-immunoreactive cells and fibers was examined in adult male Sprague-Dawley rats with and without colchicine pretreatment. As seems to be the case for a number of other neuropeptides, AII is preferentially found in brain stem, hypothalamic, and limbic structures involved in the control of homeostatic functions. AII-stained cell bodies were most prominent in magnocellular parts of the paraventricular and supraoptic nuclei, and cells were also found in parvocellular parts of the former. Other hypothalamic nuclei containing cell bodies include the suprachiasmatic nucleus, the medial preoptic area, and perifornical parts of the lateral hypothalamic area. Of considerable interest was robust staining in several of the circumventricular organs, in particular the subfornical organ, where both cells and fibers were found. The results of water deprivation and nephrectomy suggest that this staining does not represent uptake of circulating peptide, but instead, represents AII-containing neural connections. In the thalamus, AII-stained cells were found in the paraventricular nucleus, the central medial nucleus, the nucleus reuniens, and rostral parts of the zona incerta. Two cell groups in the basal telencephalon, in the dorsal part of the bed nucleus of the stria teminalis and in the medial nucleus of the amygdala, lay at either end of an AII-stained pathway coursing through the stria terminalis. In the midbrain, immunoreactive cells were found in the interpeduncular and peripeduncular nuclei, and one pontine cell group was detected in the most lateral part of the lateral parabrachial nucleus. The only AII-stained cells in the medulla were in the nucleus of the solitary tract, near the margin of the area postrema. Fibers were found at all levels of the central nervous system, from the olfactory bulbs to the spinal cord, where terminal fields were observed in the substantia gelatinosa and in the intermediolateral cell column. Longitudinally oriented fibers were present throughout the periventricular fiber system and in the medial forebrain bundle, including its caudal extension in ventrolateral parts of the brain stem. It is suggested that, at many different levels, AIIserves as both a hormone and neurotransmitter for fluid balance.