Global Ethanol-Induced Enhancements of Monoaminergic Neurotransmission: A Meta-Analysis Study
Global Ethanol-Induced Enhancements of Monoaminergic Neurotransmission: A Meta-Analysis Study
复制标题
DOI：
10.1111/acer.12207
复制
发表时间：
2013-12-01
影响因子：
3.2
通讯作者：
Noori, Hamid R.
中科院分区：
文献类型：
--
作者：
Brand, Ines;Fliegel, Sarah;Noori, Hamid R.
背景许多研究使用体内微透析作为定量方法来研究急性和慢性给予乙醇（EtOH）后不同物种特定脑区域细胞外神经递质浓度的动态变化。这些调查的一个主要焦点是乙醇诱导的影响前脑区域的神经化学，特别是剂量依赖性的神经适应性变化的单胺systems.MethodsHere，我们进行了荟萃分析发表的数据集在体内微透析测量，以评估浓度依赖性影响乙醇对单胺水平在19个不同的大脑区域在成年大鼠，其被鉴定为用于模拟药物作用的神经回路的主要成分。总共，210篇研究文章（7,407大鼠）的数据集进行了analysed.ResultsThe分析的基础值的去甲肾上腺素，血清素，多巴胺在这些地区几乎没有任何依赖性的性别，应变，或意识状态。然而，急性给予EtOH（腹腔内0.25至2.5g/kg）似乎使单胺水平整体增加，且与脑部位无关，高达基础浓度的270%。此外，约40分钟的峰值时间平均值表明最大240分钟长度的最佳时间间隔，以在微透析实验的框架内完全研究不同剂量的EtOH的影响。分析进一步显示，局部细胞外单胺浓度（峰值%基线）和EtOH的应用剂量，而浓度中EtOH诱导峰的时间出现（高峰时段）结论我们的研究结果提供了一个通用的数据库和框架，为未来的体内微透析和计算机实验的优化设计，神经化学和药理学。
BackgroundNumerous studies use in vivo microdialysis as a quantification method for studying dynamical alterations of extracellular neurotransmitter concentrations in specific brain regions of various species following acute and chronic administration of ethanol (EtOH). A major focus of these investigations is the EtOH-induced effects on the neurochemistry of forebrain regions, particularly dose-dependent neuroadaptive changes of monoamine systems.MethodsHere, we performed a meta-analysis on published data sets of in vivo microdialysis measurements to assess the concentration-dependent effects of EtOH on monoamine levels within 19 distinct brain regions in adult rats, which were identified as major components of a neurocircuitry for modeling drug effects. In total, data sets of 210 research articles (7,407 rats) were analyzed.ResultsThe analysis of the basal values of noradrenaline, serotonin, and dopamine in those regions indicated hardly any dependencies on gender, strain, or state of consciousness. However, the acute administrations of EtOH (intraperitoneal 0.25 to 2.5g/kg) appear to increase the level of monoamines globally and independent of the brain sites up to 270% of the basal concentrations. Moreover, a peak time average of approximately 40minutes suggests an optimal time interval of maximal 240minutes length to completely study the effects of different doses of EtOH within the framework of microdialysis experiments. The analysis further revealed a positive correlation between the magnitude of increase (peak % baseline) of local extracellular monoamine concentrations and the applied doses of EtOH, while the temporal occurrence of the EtOH-induced peaks in the concentrations (peak time) was mostly negatively correlated.ConclusionsOur results provide a universal database and framework for the optimal design of future in vivo microdialysis and in silico experiments in neurochemistry and pharmacology.