Potentials of the elevated circulating miR-185 level as a biomarker for early diagnosis of HBV-related liver fibrosis
Potentials of the elevated circulating miR-185 level as a biomarker for early diagnosis of HBV-related liver fibrosis
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循环中升高的 miR-185 水平作为早期诊断 HBV 相关肝纤维化的生物标志物的潜力
DOI：
10.1038/srep34157
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发表时间：
2016-09-28
影响因子：
4.6
通讯作者：
Zhu, Liang
中科院分区：
文献类型：
--
作者：
Li, Bin-bin;Li, Dong-liang;Zhu, Liang
肝纤维化的早期诊断对于各种慢性肝病的早期干预和预后至关重要。传统的重复组织学评估是不切实际的，由于相关的侵入性。在目前的研究中，我们评估了循环miR-185作为预测肝纤维化发生和进展的潜在生物标志物。我们发现miR-185在HBV感染肝纤维化患者和肝纤维化大鼠的血液标本中显著上调，miR-185水平与肝纤维化进展相关，但与HBV感染患者的不同病毒载量无关。在晚期肝纤维化过程中，在胶原沉积区域观察到miR-185。我们发现，miR-185水平的差异有助于区分早期或晚期肝纤维化和健康对照组，具有高特异性，灵敏度和似然比，使用接受者-操作者特征分析。miR-185靶向SREBF 1，并增加作为肝纤维化标志的COL 1A 1和a-SMA基因的表达。我们的数据支持循环miR-185水平可用作肝纤维化早期诊断的潜在生物标志物。
Early diagnosis of liver fibrosis is critical for early intervention and prognosis of various chronic liver diseases. Conventional repeated histological assessment is impractical due to the associated invasiveness. In the current study, we evaluated circulating miR-185 as a potential biomarker to predict initiation and progression of liver fibrosis. We found that miR-185 was significantly up-regulated in blood specimens from patients with HBV-liver fibrosis and rats with liver fibrosis, the miR-185 levels were correlated with liver fibrosis progression, but not with the different viral loads in HBV-infected patients. miR-185 was observed in collagen deposition regions during advanced liver fibrosis. We found that differences in miR-185 levels facilitated the discrimination between early-staged or advanced-staged liver fibrosis and the healthy controls with high specificity, sensitivity, and likelihood ratio using receiver-operator characteristic analysis. miR-185 targeted SREBF1, and increased expression of COL1A1 and a-SMA genes that are hallmarks of liver fibrosis. Our data supported that circulating miR-185 levels could be used as potential biomarkers for the early diagnosis of liver fibrosis.