Adipo/cytokines in atherosclerotic secretomes: increased visfatin levels in unstable carotid plaque.
Adipo/cytokines in atherosclerotic secretomes: increased visfatin levels in unstable carotid plaque.
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DOI：
10.1186/s12872-016-0320-5
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发表时间：
2016-07-08
影响因子：
2.1
通讯作者：
Richart C
中科院分区：
文献类型：
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作者：
Auguet T;Aragonès G;Guiu-Jurado E;Berlanga A;Curriu M;Martinez S;Alibalic A;Aguilar C;Camara ML;Hernández E;Ruyra X;Martín-Paredero V;Richart C
新的促炎和抗炎衍生物从脂肪组织，称为脂肪因子，作为代谢因子。本研究的目的是分析不稳定颈动脉粥样硬化斑块与非动脉粥样硬化乳腺动脉分泌物中不同脂肪/细胞因子的分泌表达。我们通过ELISA评估了来自培养的不稳定颈动脉粥样硬化斑块（n = 18）和非动脉粥样硬化乳腺动脉（n = 13）的人分泌物组中脂联素、内脂素、脂质运载蛋白-2（lipocalin-2）、TNF-α、IL-6和TNFR 2的分泌水平。我们还测量了动脉粥样硬化患者和健康受试者血清队列（n = 16）中的内脂素血清水平。我们发现，内脂素水平显着增加不稳定的颈动脉粥样硬化斑块分泌组比非动脉粥样硬化乳腺动脉分泌组。关于所研究的其他adipo/细胞因子，未发现差异。内脂素循环水平在颈动脉粥样硬化不稳定斑块组和非粥样硬化乳腺动脉组之间无差异。然而，与健康受试者的血清队列相比，这些内脂素水平升高。在分析的所有脂肪/细胞因子中，只有内脂素在不稳定的颈动脉粥样硬化斑块的分泌物中显示出水平增加。需要进一步的人体研究来阐明内脂素作为不稳定颈动脉粥样硬化斑块的预后因子的可能作用。
Novel pro-inflammatory and anti-inflammatory derivatives from adipose tissue, known as adipokines, act as metabolic factors. The aim of this study was to analyse the secreted expression of different adipo/cytokines in secretomes of unstable carotid atherosclerotic plaque versus non-atherosclerotic mammary artery. We evaluated the secretion levels of adiponectin, visfatin, lipocalin-2, resistin, IL-6 and TNFR2 by ELISA in human secretomes from cultured unstable carotid atherosclerotic plaque (n = 18) and non-atherosclerotic mammary artery (n = 13). We also measured visfatin serum levels in patients suffering from atherosclerosis and in a serum cohort of healthy subjects (n = 16). We found that visfatin levels were significantly increased in unstable carotid atherosclerotic plaque secretome than in non-atherosclerotic mammary artery secretome. No differences were found with regard the other adipo/cytokines studied. Regarding visfatin circulating levels, there were no differences between unstable carotid atherosclerotic plaque and non-atherosclerotic mammary artery group. However, these visfatin levels were increased in comparison to serum cohort of healthy subjects. Of all the adipo/cytokines analysed, only visfatin showed increased levels in secretomes of unstable carotid atherosclerotic plaque. Additional human studies are needed to clarify the possible role of visfatin as prognostic factor of unstable carotid atherosclerotic plaque.