Haemolytic activities and adjuvant effect of Bupleurum chinense saponins on the immune responses to ovalbumin in mice.
Haemolytic activities and adjuvant effect of Bupleurum chinense saponins on the immune responses to ovalbumin in mice.
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DOI：
10.1016/j.vaccine.2005.09.030
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发表时间：
2006-02
期刊：
影响因子：
5.5
通讯作者：
Hongxiang Sun
中科院分区：
文献类型：
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作者：
Hongxiang Sun
本研究评价了柴胡总皂甙(BCS)的溶血活性及其对ICR小鼠卵白蛋白(OVA)免疫应答的佐剂作用。牛血清白蛋白具有轻微的溶血作用，在浓度为500和250μg/ml时，其溶血率分别为3.32%和1.19%。分别于第1天和第15天分别用卵清蛋白100μg和溶于含有氢氧化铝凝胶(Alum200μg)、奎尼拉(10和20μg)或牛血清白蛋白(50、100或200μg)的生理盐水中的卵清蛋白100μg皮下免疫小鼠。2周后(第28天)，检测刀豆蛋白A(ConA)、脂多糖(LPS)和卵清蛋白(OVA)刺激的脾细胞增殖反应和血清中OVA特异性抗体。牛血清白蛋白可显著促进刀豆蛋白A、内毒素和卵清蛋白诱导的小鼠脾细胞增殖，尤以100μg剂量最明显(P<；0.05或P<；0.001)。血清中卵特异性Ig G、Ig G1和Ig G2b抗体水平也显著高于卵清蛋白对照组(P<；0.01或P<；0.001)。此外，BCS和Quila对小鼠OVA特异性IgG2b抗体应答的增强作用无显著差异(P>；0.05)。综上所述，BCS可安全用作低溶血或无溶血作用的佐剂。
In this study, the haemolytic activities of Bupleurum chinense saponins (BCS) and its adjuvant potentials on the immune responses of ICR mice against ovalbumin (OVA) were evaluated. BCS showed a slight haemolytic effect, with its haemolytic percents being 3.32% and 1.19% at the concentrations of 500 and 250μg/ml, respectively. ICR mice were immunized subcutaneously with OVA 100μg alone or with OVA 100μg dissolved in saline containing aluminium hydroxide gel (Alum, 200μg), QuilA (10 and 20μg) or BCS (50, 100 or 200μg) on Days 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. BCS significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice especially at a dose of 100μg (P<0.05 or P<0.001). OVA-specific IgG, IgG1 and IgG2b antibody levels in serum were also significantly enhanced by BCS compared with OVA control group (P<0.01 or P<0.001). Moreover, no significant differences (P>0.05) were observed between enhancing effect of BCS and QuilA on the OVA-specific IgG2b antibody responses to OVA in mice. In conclusion, the results suggest that BCS could be safely used as adjuvant with low or non-haemolytic effect.