Production of reactive oxygen species by hemocytes of Biomphalaria glabrata:: carbohydrate-specific stimulation
Production of reactive oxygen species by hemocytes of Biomphalaria glabrata:: carbohydrate-specific stimulation
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DOI：
10.1016/s0145-305x(00)00017-3
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Bayne, CJ
中科院分区：
文献类型：
--
作者：
Hahn, UK;Bender, RC;Bayne, CJ
识别特定的碳水化合物结构，通常发生在入侵病原体的表面，被认为是引发无脊椎动物的内部防御机制。为了研究碳水化合物的性质，引起防御反应的腹足类Biomphalaria光滑的血细胞，我们测试了八种不同的碳水化合物，共轭牛血清白蛋白（BSA），产生活性氧（ROS）。六个测试的碳水化合物部分被认为存在于S上。曼氏孢子囊表面的碳水化合物（甘露糖、半乳糖、岩藻糖、N-乙酰基-葡糖胺、N-乙酰基-半乳糖胺和乳糖）;其他两种测试的碳水化合物是葡萄糖和蜜二糖。使用荧光探针-2 '，7'-二氯荧光素-二乙酸酯（DCFH-DA）测量ROS产生。血细胞来源于两种不同的B菌株。光滑：所用菌株之一（MO）对曼氏血吸虫（PR-1菌株）的感染敏感，而另一种蜗牛菌株（13-16-R1）对PR-1的感染具有抗性。三种BSA-碳水化合物结合物（BSA-半乳糖、BSA-甘露糖和BSA-岩藻糖）。刺激软体动物血细胞中活性氧的产生。血细胞的反应是相似的，无论是来自敏感或抗性蜗牛。如果我们发现的碳水化合物结构，刺激活性氧产生参与寄生虫识别，我们的研究结果表明，寄生虫杀死可能涉及活性氧产生的定性差异，或其他因素。(C)2000爱思唯尔科技有限公司版权所有。
Recognition of specific carbohydrate structures, which occur commonly on the surfaces of invading pathogens, is thought to elicit internal defense mechanisms in invertebrates. To investigate the nature of carbohydrates that evoke a defensive response in hemocytes of the gastropod Biomphalaria glabrata, we tested eight different carbohydrates, conjugated to bovine serum albumin (BSA), for generation of reactive oxygen species (ROS). Six of the carbohydrate moieties tested are thought to be present on the S. mansoni sporocyst surface (mannose, galactose, fucose, N-acetyl-glucosamine, N-acetyl-galactosamine, and lactose); the other two carbohydrates tested were glucose and melibiose. ROS generation was measured using the fluorescent probe - 2',7'-dichlorofluorescein-diacetate (DCFH-DA). Hemocytes were derived from two different strains of B. glabrata: one of the strains used (MO) is susceptible to infection by the trematode Schistosoma mansoni (PR-1 strain), while the other snail strain (13-16-R1) is resistant to infection with PR-1. Three of the BSA-carbohydrate conjugates (BSA-galactose, BSA-mannose, and BSA-fucose). stimulated generation of reactive oxygen species in the molluscan hemocytes. The responses of the hemocytes were similar whether they were derived from susceptible or resistant snails. If the carbohydrate structures we found, to stimulate ROS generation are involved in parasite recognition, our results suggest that parasite killing may involve either qualitative differences in production of reactive oxygen species, or additional factors. (C) 2000 Elsevier Science Ltd. All rights reserved.