Intramitochondrial pH and Intra-extramitochondrial pH Gradient of Beef Heart Mitochondria in Various Functional States
Intramitochondrial pH and Intra-extramitochondrial pH Gradient of Beef Heart Mitochondria in Various Functional States
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DOI：
10.1038/214400b0
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发表时间：
1967-04
期刊：
影响因子：
64.8
通讯作者：
S. Addanki;F. D. Cahill;J. F. Sotos
中科院分区：
文献类型：
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作者：
S. Addanki;F. D. Cahill;J. F. Sotos
5，5-二甲基2，4-恶唑烷二酮（DMO）是一种弱惰性酸（37° C时pKa6.13），已用于根据其在肌肉组织和血浆之间的分布计算肌肉细胞内pH值1。虽然有相当大的兴趣，离子易位，氢离子分泌和膜pH梯度的线粒体，一种方法来测量intraoproteinalpH值的绝对值是不可用的。测量脑内pH值将大大有助于了解线粒体功能。因此，DMO被用来测量牛心脏线粒体在静息，呼吸（状态4），呼吸和磷酸化（状态3），和钙离子（II）激活的呼吸状态下的线粒体内和线粒体外的DMO的分布的基础上intraperialpH和intra-extraperialpH梯度。
5,5-DIMETHYL 2,4-oxazolidinedione (DMO), a weak inert acid (pKa6.13 at 37° C), has been employed to calculate the muscle intracellularpH based on its distribution between the muscle tissue and plasma1. Although there is considerable interest in ion translocations, hydrogen ion secretion and membranepH gradient of mitochondria, a method for the measurement of absolute values of intramitochondrialpH is not available. Measurement of intramitochondrialpH would greatly contribute to the understanding of mitochondrial function. DMO was therefore used to measure the intramitochondrialpH and intra-extramitochondrialpH gradient of beef heart mitochondria in resting, respiring (state 4), both respiring and phosphorylating (state 3), and calcium ion(II) activated respiratory states on the basis of the intra- and extra-mitochondrial distribution of DMO.