Activated air produced by shielded sliding discharge plasma mediates plasmid DNA delivery to mammalian cells
Activated air produced by shielded sliding discharge plasma mediates plasmid DNA delivery to mammalian cells
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DOI：
10.1002/bit.25660
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发表时间：
2015-12-01
影响因子：
3.8
通讯作者：
Heller, Richard
中科院分区：
文献类型：
--
作者：
Edelblute, Chelsea M.;Heller, Loree C.;Heller, Richard
冷等离子体正在成为一种潜在的医学应用方法。目前的研究评估了一种新的冷等离子体反应器的有效性的基础上屏蔽滑动放电产生阴极导向的流光在环境空气中产生的质粒DNA的交付。使用接种编码荧光素酶的质粒DNA的小鼠黑色素瘤细胞（B16 F10）和人角质形成细胞（HaCaT）进行实验。在72小时内测量的定量结果显示，暴露于等离子体活化空气（PAA）的细胞中的荧光素酶表达水平比单独接种质粒DNA获得的水平高5倍（P
Cold plasma is emerging as a potential method for medical applications. The current study assessed the efficacy of a novel cold plasma reactor based on shielded sliding discharge producing cathode-directed streamers generated in ambient air for the delivery of plasmid DNA. Experiments were performed with mouse melanoma cells (B16F10) and human keratinocyte cells (HaCaT) inoculated with plasmid DNA encoding luciferase. Quantitative results measured over a 72-h period displayed luciferase expression levels as high as 5-fold greater in cells exposed to plasma-activated air (PAA) than levels obtained from the inoculation of plasmid DNA alone (P