Clonal replacement of epidemic methicillin-resistant Staphylococcus aureus strains in a German university hospital over a period of eleven years.
Clonal replacement of epidemic methicillin-resistant Staphylococcus aureus strains in a German university hospital over a period of eleven years.
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DOI：
10.1371/journal.pone.0028189
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Monecke S
中科院分区：
文献类型：
--
作者：
Albrecht N;Jatzwauk L;Slickers P;Ehricht R;Monecke S
在世界范围内，耐甲氧西林金黄色葡萄球菌（MRSA）增加了医疗保健和社区相关感染的风险。自1961年在英国首次报道MRSA以来，已经出现了几个不同的克隆或菌株。在整个国家、小区域或单个医院的MRSA人群中观察到了一些克隆取代其他克隆的变化。在这项研究中，根据对778株分离株和每年至少50株MRSA的微阵列分析，描述了2000年至2010年期间德国东南部三级保健医院MRSA分离株的克隆替代。在这11年中，鉴定了四种常见的流行菌株，CC 22-MRSA-IV、CC 45-MRSA-IV、CC 5/ST 228-MRSA-I（包括具有截短SCCmec元件的变体）和CC 5-MRSA-II。2001年首次检测到PVL阴性CC 22-MRSA-IV菌株（Barnim流行株，UK-EMRSA-15），此后其丰度增加至2010年的58.6%。CC 5-MRSA-II从2%（2000年）增加到约30%（2003年），此后在23%至37%的分离株之间波动。CC 5/ST 228-MRSA-I从约一半的检测分离株（2000年）下降至2.3%（2010年）。CC 45-MRSA-IV也观察到类似的趋势，在2002年达到最高值62.0%后，2010年急剧下降至3.4%。另外17种PVL阴性MRSA菌株被零星发现，未观察到显著趋势。发现了7株PVL阳性MRSA菌株，但在研究期间它们仍然罕见，占分离株的2.7%。
Worldwide, methicillin-resistant Staphylococcus aureus (MRSA) pose an increased risk for healthcare- and community-associated infections. Since the first report of MRSA in England in 1961, several distinct clones or strains have emerged. Changes within the MRSA population of whole countries, small regions or of single hospitals have been observed with some clones replacing others. In this study, the clonal replacement of MRSA isolates in a South-eastern German tertiary care hospital between 2000 and 2010 is described based on microarray analyses of 778 isolates and at least 50 MRSA per year. Within these eleven years, four common epidemic strains, CC22-MRSA-IV, CC45-MRSA-IV, CC5/ST228-MRSA-I (including a variant with a truncated SCCmec element) and CC5-MRSA-II were identified. The PVL-negative CC22-MRSA-IV strain (Barnim Epidemic Strain, UK-EMRSA-15) was detected for the first time in 2001 and its abundance increased since then to 58.6% in 2010. CC5-MRSA-II increased from 2% (2000) to about 30% (2003), and since then it fluctuates between 23 and 37% of isolates. CC5/ST228-MRSA-I decreased from about the half of tested isolates (2000) to 2.3% (2010). A similar trend was observed for CC45-MRSA-IV, which decreased drastically down to 3.4% in 2010 after reaching a maximum of 62.0% in 2002. Seventeen other PVL-negative MRSA strains were identified sporadically with no significant trend being observed. Seven PVL-positive MRSA strains were found, but they remained rare during the study period accounting together for 2.7% of isolates.
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影响因子：
14.2
作者：
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通讯作者：
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