MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS.
MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS.
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DOI：
10.1038/s41598-018-31844-1
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发表时间：
2018-09-12
影响因子：
4.6
通讯作者：
Lauc G
中科院分区：
文献类型：
--
作者：
Zaytseva OO;Jansen BC;Hanić M;Mrčela M;Razdorov G;Stojković R;Erhardt J;Brizić I;Jonjić S;Pezer M;Lauc G
Immunoglobulin G (IgG) N-glycosylation is crucial for its effector functions. It is a complex trait, and large sample sets are needed to discover multiple genetic factors that underlie it. While in humans such high-throughput studies of IgG N-glycans became usual, only one has been carried out in mice. Here we describe and validate a method for the relative quantification of IgG Fc-linked N-glycans in a subclass-specific manner using nano-reverse phase liquid chromatography coupled with mass-spectrometry (nanoRP-LC-MS) applied to murine IgG. High-throughput data processing is ensured by the LaCyTools software. We have shown that IgG isolation procedure is the main source of technical variation in the current protocol. The major glycoforms were quantified reliably with coefficients of variation below 6% for all the analytes with relative abundances above 5%. We have applied our method to a sample set of 3 inbred strains: BALB/c, C57BL/6 and C3H and observed differences in subclass-specific and strain-specific N-glycosylation of IgG, suggesting a significant genetic component in the regulation of Fc-linked IgG N-glycosylation.
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影响因子：
7.4
作者：
Bones, Jonathan;Mittermayr, Stefan;Rudd, Pauline M.
通讯作者：
Rudd, Pauline M.
DOI：
10.1074/mcp.m113.037465
发表时间：
2014-06
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Huffman JE;Pučić-Baković M;Klarić L;Hennig R;Selman MH;Vučković F;Novokmet M;Krištić J;Borowiak M;Muth T;Polašek O;Razdorov G;Gornik O;Plomp R;Theodoratou E;Wright AF;Rudan I;Hayward C;Campbell H;Deelder AM;Reichl U;Aulchenko YS;Rapp E;Wuhrer M;Lauc G
通讯作者：
Lauc G
DOI：
10.1073/pnas.91.16.7782
发表时间：
1994-08-02
影响因子：
11.1
作者：
KLEIN, A;KRISHNA, M;VARKI, A
通讯作者：
VARKI, A
影响因子：
64.5
作者：
DiLillo DJ;Ravetch JV
通讯作者：
Ravetch JV
影响因子：
2.2
作者：
Mahan, Alison E.;Tedesco, Jacquelynne;Alter, Galit
通讯作者：
Alter, Galit