An SNP marker at the STAT6 locus can identify the hybrids between rhesus (Macaca mulatta) and long-tailed macaques (M. fascicularis) in Thailand: a rapid and simple screening method and its application.
An SNP marker at the STAT6 locus can identify the hybrids between rhesus (Macaca mulatta) and long-tailed macaques (M. fascicularis) in Thailand: a rapid and simple screening method and its application.
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STAT6位点的SNP标记可识别泰国恒河猴（Macaca mulatta）和长尾猕猴（M. fasciculis）的杂交种：一种快速简便的筛选方法及其应用。
DOI：
10.1007/s10329-015-0502-2
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发表时间：
2016
期刊：
影响因子：
1.7
通讯作者：
Malaivijitnond S
中科院分区：
文献类型：
--
作者：
Jadejaroen J;Kawamoto Y;Hamada Y;Malaivijitnond S
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对恒河猴（Macaca mulatta）和长尾猴（M. fascicularis）进行了遗传区分。利用apai对STAT6基因座的745 bp扩增子进行酶切和电泳，得到(1)2条（234和511 bp）、(2)1条（745 bp）和(3)3条（234、511和745 bp）基因型，分别对应于G/G（长尾猕猴特异性纯合子）、a / a（恒河猕猴特异性纯合子）和a /G（杂交特异性杂合子）基因型。在泰国野生恒河猴和长尾猕猴以及已知杂交种群中测试了该PCR-RFLP方法的诊断稳健性和效率。印度支那和Sundaic长尾猕猴样本（n= 18）均为纯合G/G型，印度支那恒河猴样本（n= 10）均为纯合a / a型。Khao Khieow开放动物园的恒河猴/长尾杂交种群是由20年前引进的恒河猴与本地长尾猕猴杂交而成的，其中47%（56/118）的样本具有异质A/G基因型。此外，在2006-2014年期间，杂交种群中恒河猴特异性等位基因A的频率显著下降，其中检测到STAT6基因型与个体形态之间存在强烈关联。总之，一种强大的PCR-RFLP分析方法可以提供一种简单、有效和廉价的方法来评估恒河猴和长尾猕猴之间的杂交个体，特别是在实地研究中。虽然该方法不能确定两代或两代以上的所有杂交，但它至少可以对杂交过程进行评估，因此它适用于评估两个物种在其地理范围内的自然或人为杂交状况。
A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) assay was developed to genetically discriminate rhesus (Macaca mulatta) macaques from long-tailed (M. fascicularis) macaques. The 745 bp PCR amplicon of the STAT6 locus that spans a potentially species-diagnostic single nucleotide polymorphism (SNP) marker was digested withApaI and gel electrophoresed to give (1) two (234 and 511 bp), (2) one (745 bp) and (3) three (234, 511 and 745 bp) band patterns that correspond to the genotypes G/G (long-tailed macaque specific homozygote), A/A (rhesus macaque specific homozygote) and A/G (hybrid specific heterozygote), respectively. The diagnostic robustness and efficiency of this PCR–RFLP assay was tested on wild rhesus and long-tailed macaques inhabiting Thailand and a known hybrid population. The Indochinese and Sundaic long-tailed macaque samples (n= 18) all showed a homozygous G/G pattern, while the Indochinese rhesus macaques (n= 10) all showed a homozygous A/A pattern. The rhesus/long-tailed hybrid population at Khao Khieow Open Zoo, which resulted from an introduced group of rhesus macaques that hybridized with the indigenous long-tailed macaques about 20 years ago, revealed 47 % (56/118 samples analyzed) with the heterogenous A/G genotype. In addition, the frequency of the rhesus-specific allele A significantly decreased in the hybrid population during 2006–2014, where a strong association between the STAT6 genotype and the morphology of the individuals was detected. In conclusion, a robust PCR–RFLP assay allows a simple, effective and inexpensive approach, in particular for field studies, to assess hybrid individuals between rhesus and long-tailed macaques. Although this assay cannot conclusively identify all the hybrids over two or more generations, it at least can allow the evaluation of the process of hybridization, and so it is applicable to the assessment of the status of natural or anthropogenic hybridization between the two species across their geographic range.