The effects of PEG grafting level and injection dose on gold nanorod biodistribution in the tumor-bearing mice
The effects of PEG grafting level and injection dose on gold nanorod biodistribution in the tumor-bearing mice
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DOI：
10.1016/j.jconrel.2009.06.006
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发表时间：
2009-10-01
影响因子：
10.8
通讯作者：
Niidome, Takuro
中科院分区：
文献类型：
--
作者：
Akiyama, Yasuyuki;Mori, Takeshi;Niidome, Takuro
金纳米棒在近红外区域有很强的吸收性，可以深入渗透到组织中，吸收的光能转化为热能。因此，金纳米棒有望作为体内生物成像的有效造影剂和光热疗法的热转换器。我们将不同量的聚乙二醇（PEG）接枝到金纳米棒表面，并研究接枝水平和注射剂量对静脉注射和增强渗透性和保留（EPR）后荷瘤小鼠体内生物分布的影响。较高的 PEG 接枝水平有利于避免网状内皮系统 (RES) 和抑制金纳米棒在循环中的聚集。 PEG:金摩尔比为 1.5 的修饰足以显示延长的循环和 EPR 效果。当注射剂量增加到19.5μg金以上时，肝脏中RES的摄取达到饱和，多余的金纳米棒分布到其他组织，特别是脾脏和肿瘤。该研究结果将为金纳米棒光热效应介导的癌症疗法的发展提供重要基础。 (C) 2009 Elsevier B.V. 保留所有权利。
Gold nanorods have strong absorbance in the near infrared region, which penetrates deeply into tissues, where the absorbed light energy is converted into heat. Therefore, gold nanorods are expected to act as an effective contrast agent for in vivo bioimaging and as a thermal converter for photothermal therapy. We grafted various amounts of polyethylene glycol (PEG) onto the surface of gold nanorods and investigated the effects of grafting level and injection dose on the biodistribution in the tumor-bearing mice after intravenous injection and enhanced permeability and retention (EPR). Higher PEG grafting levels were advantageous for reticuloendothelial system (RES) avoidance and for suppression of aggregation of the gold nanorods in the circulation. Modification with a PEG:gold molar ratio of 1.5 was sufficient to show both prolonged circulation and the EPR effect. When the injection dose was increased above 19.5 mu g of gold, the RES uptake in the liver was saturated and surplus gold nanorods were distributed to other tissues, especially the spleen and the tumor. The results of this study will provide an important basis for the development of cancer therapies mediated by the photothermal effect of gold nanorods. (C) 2009 Elsevier B.V. All rights reserved.