Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing.
Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing.
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DOI：
10.1007/s41048-015-0007-y
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发表时间：
2015
影响因子：
--
通讯作者：
Bulleid NJ
中科院分区：
文献类型：
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作者：
Cao Z;Bulleid NJ
典型的2-半胱氨酸过氧化物氧还蛋白（Prxs）的活性可以通过过氧化调节，从而导致氧化还原活性的丧失。在这里，我们开发了一种简单的检测方法，以监测不同的典型2-半胱氨酸prxs的过氧化水平同时。该分析仅需要标准设备，并且可以比较相同凝胶上的不同样品。它比传统的2D凝胶需要更少的时间，并且比使用过氧化氧还蛋白特异性抗体的Western印迹法提供更多的信息。该方法也可用于监测具有电荷差异的蛋白质修饰，例如磷酸化。
The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously. This assay only requires standard equipment and can compare different samples on the same gel. It requires much less time than conventional 2D gels and gives more information than Western blotting with an antibody specific for hyperoxidized peroxiredoxin. This method could also be used to monitor protein modification with a charge difference such as phosphorylation.