Leachates from municipal solid waste disposal sites harbor similar, novel nitrogen-cycling bacterial communities.
Leachates from municipal solid waste disposal sites harbor similar, novel nitrogen-cycling bacterial communities.
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DOI：
10.1111/j.1574-6968.2006.00560.x
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发表时间：
2007-02
影响因子：
2.1
通讯作者：
Shuang Zhu;G. Chan;Kaiyun Cai;L. Qu;Li-Nan Huang
中科院分区：
文献类型：
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作者：
Shuang Zhu;G. Chan;Kaiyun Cai;L. Qu;Li-Nan Huang
近年来，在城市固体废物（MSW）填埋场，一氧化二氮（N（2）O）的排放量很高。然而，所涉及的细菌种群的生物多样性仍未被探索。在这项研究中，我们调查了社区的氨氧化细菌（AOB）和硝化细菌与渗滤液从三个城市生活垃圾处置场通过检查氨单加氧酶结构基因amoA和一氧化二氮还原酶基因nosZ的多样性，分别。功能基因的克隆和系统发育分析揭示了新的和相似的原核生物群参与不同化学组成的渗滤液中的氮循环。所有的amoA序列回收分组内的亚硝化单胞菌欧洲集群中的β-变形菌，绝大多数只显示出相对温和的序列相似性，已知的AOB，但完全最相似的环境克隆以前从废水处理厂检索。检索到的所有nosZ序列没有与迄今为止报道的任何nosZ基因聚类，并且仅与来自γ-变形菌门的公认的杀菌剂远程相关，因此不能隶属。发现三个反硝化nosZ渗滤液群落有明显的重叠。我们的研究表明，在这些城市生活垃圾处置场的独特环境中的新型N-循环基团的显着选择。
High emissions of nitrous oxide (N(2)O) have recently been documented at municipal solid waste (MSW) landfills. However, the biodiversity of the bacterial populations involved remains unexplored. In this study, we investigated communities of ammonia-oxidizing bacteria (AOB) and denitrifying bacteria associated with the leachates from three MSW disposal sites by examining the diversity of the ammonia monooxygenase structural gene amoA and the nitrous oxide reductase gene nosZ, respectively. Cloning and phylogenetic analysis of the functional genes revealed novel and similar groups of prokaryotes involved in nitrogen cycling in the leachates with different chemical compositions. All amoA sequences recovered grouped within the Nitrosomonas europaea cluster in the Betaproteobacteria, with the vast majority showed only relatively moderate sequence similarities to known AOB but were exclusively most similar to environmental clones previously retrieved from wastewater treatment plants. All nosZ sequences retrieved did not cluster with any hitherto reported nosZ genes and were only remotely related to recognized denitrifiers from the Gammaproteobacteria and thus could not be affiliated. Significant overlap was found for the three denitrifying nosZ leachate communities. Our study suggests a significant selection of the novel N-cycling groups by the unique environment at these MSW disposal sites.