Microgrooved Polymer Substrates Promote Collective Cell Migration To Accelerate Fracture Healing in an in Vitro Model.
Microgrooved Polymer Substrates Promote Collective Cell Migration To Accelerate Fracture Healing in an in Vitro Model.
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DOI：
10.1021/acsami.5b07976
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发表时间：
2015-10-21
影响因子：
9.5
通讯作者：
Mao C
中科院分区：
文献类型：
--
作者：
Zhang Q;Dong H;Li Y;Zhu Y;Zeng L;Gao H;Yuan B;Chen X;Mao C
表面形貌可以影响细胞粘附、形态、极性、细胞骨架组织和成骨。然而，对于修复骨不连和大骨缺损时地形对骨折愈合的影响知之甚少。骨植入物表面的微槽形貌可以促进细胞迁移到骨折间隙中，从而加速骨折愈合。为了证明这一假设，我们对微槽聚己内酯基底进行了体外骨折（伤口）愈合测定，以研究微槽宽度和深度对成骨细胞样细胞（MG-63）迁移和随后愈合的影响。我们发现，与平坦基底相比，微槽基底促进 MG-63 细胞沿着凹槽和脊集体迁移到伤口间隙中，作为断裂模型。此外，凹槽宽度对伤口愈合没有显示出明显的影响，而较小的凹槽深度往往有利于集体细胞迁移，从而有利于随后的愈合。微槽基质通过促进集体细胞迁移到伤口间隙而不是通过促进细胞增殖来加速伤口愈合。此外，还发现微槽可以促进人间充质干细胞（hMSC）的迁移以治愈骨折模型。虽然 hMSC 的成骨分化在微槽基质上没有得到改善，但 hMSC 沉积的胶原蛋白 I 和矿物质的组织方式与天然骨细胞外基质中的组织方式相似。这些发现表明有必要使用微槽植入物来增强骨修复中的骨折愈合。
Surface topography can affect cell adhesion, morphology, polarity, cytoskeleton organization, and osteogenesis. However, little is known about the effect of topography on the fracture healing in repairing nonunion and large bone defects. Microgrooved topography on the surface of bone implants may promote cell migration into the fracture gap to accelerate fracture healing. To prove this hypothesis, we used an in vitro fracture (wound) healing assay on the microgrooved polycaprolactone substrates to study the effect of microgroove widths and depths on the osteoblast-like cell (MG-63) migration and the subsequent healing. We found that the microgrooved substrates promoted MG-63 cells to migrate collectively into the wound gap, which serves as a fracture model, along the grooves and ridges as compared with the flat substrates. Moreover, the groove widths did not show obvious influence on the wound healing whereas the smaller groove depths tended to favor the collective cell migration and thus subsequent healing. The microgrooved substrates accelerated the wound healing by facilitating the collective cell migration into the wound gaps but not by promoting the cell proliferation. Furthermore, microgrooves were also found to promote the migration of human mesenchymal stem cells (hMSCs) to heal the fracture model. Though osteogenic differentiation of hMSCs was not improved on the microgrooved substrate, collagen I and minerals deposited by hMSCs were organized in a way similar to those in the extracellular matrix of natural bone. These findings suggest the necessity in using microgrooved implants in enhancing fracture healing in bone repair.