Characterization of the Streptomyces lividans PspA response
Characterization of the Streptomyces lividans PspA response
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DOI：
10.1128/jb.01966-07
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发表时间：
2008-05-01
影响因子：
3.2
通讯作者：
Anne, Jozef
中科院分区：
文献类型：
--
作者：
Vrancken, Kristof;Van Mellaert, Lieve;Anne, Jozef
噬菌体休克蛋白（Psp）是由细胞质外应激诱导的，可降低细胞的能量状态。它在大肠杆菌中由噬菌体休克蛋白调节子编码，该调节子由pspABCDE和pspF和pspG组成。噬菌体休克蛋白系统在大量革兰氏阴性菌中高度保守。然而，许多细菌基因组仅含有pspA同源物，但不含有Psp系统的其他基因的同源物。这种保守性表明PspA单独可能在这些细菌中发挥重要作用。在变铅青链霉菌（Streptomyces lividans）中，一种土传革兰氏阳性菌，噬菌体休克蛋白系统仅由pspA基因组成。在这份报告中，我们发现pspA编码一个28 kDa的蛋白质，该蛋白质存在于S.青灰色菌丝体我们证明了pspA基因在攻击膜完整性的应激条件下被强烈诱导，并且在大多数这些条件下对生长和存活至关重要。本文报道的数据清楚地表明PspA在S.在胁迫条件下，PspA在变铅青菌中的表达，表明PspA在大多数细菌中可能比以前认为的更重要。
Phage shock protein (Psp) is induced by extracytoplasmic stress that may reduce the energy status of the cell. It is encoded in Escherichia coli by the phage shock protein regulon consisting of pspABCDE and by pspF and pspG. The phage shock protein system is highly conserved among a large number of gram-negative bacteria. However, many bacterial genomes contain only a pspA homologue but no homologues of the other genes of the Psp system. This conservation indicates that PspA alone might play an important role in these bacteria. In Streptomyces lividans, a soil-borne gram-positive bacterium, the phage shock protein system consists only of the pspA gene. In this report, we showed that pspA encodes a 28-kDa protein that is present in both the cytoplasmic and the membrane fractions of the S. lividans mycelium. We demonstrated that the pspA gene is strongly induced under stress conditions that attack membrane integrity and that it is essential for growth and survival under most of these conditions. The data reported here clearly show that PspA plays an important role in S. lividans under stress conditions despite the absence of other psp homologues, suggesting that PspA may be more important in most bacteria than previously thought.