FOXO links wing form polyphenism and wound healing in the brown planthopper, Nilaparvata lugens
FOXO links wing form polyphenism and wound healing in the brown planthopper, Nilaparvata lugens
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FOXO 将褐飞虱 (Nilaparvata lugens) 的翅膀形态多型性和伤口愈合联系起来
DOI：
10.1016/j.ibmb.2015.12.002
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发表时间：
2016-03-01
影响因子：
3.8
通讯作者：
Lavine, Laura Corley
中科院分区：
文献类型：
--
作者：
Lin, Xinda;Yao, Yun;Lavine, Laura Corley
多型现象，如翅膀二型和种姓决定是重要的，让动物适应不断变化的环境。褐飞虱（Nilaparvata lugens）是一种严重的农业害虫，其翅型分为长翅型（macropterous）和短翅型（bracypterous）。长翅膀专门用于迁徙，而短翅膀则存在于专门用于繁殖的个体中。在研究褐飞虱翅型多型性的同时，我们从4龄褐飞虱上切下单个翅垫，以保存与成虫翅型相关的转录记录。令人惊讶的是，我们发现，切除一个翼垫从一对前翅改变了翅膀形态的另一个翅膀后，发展到成人，在短翅形态。进一步的实验表明，不仅切除或切割翼垫，而且腹部针刺都使发育成短翅成虫的比例显着增加。因此，受伤似乎会导致向短翅发育的转变。然后，我们测试了N.这是转录因子FOXO的一个新的突变体，它已被证明有助于介导褐飞虱的翅多型性和切除翅垫后小鼠的伤口愈合。在翅垫切除后，N1FOXO及其下游靶标N14 EBP均显著增加。这些结果表明，FOXO介导的翅膀发育和伤口愈合的N。lugens，这导致了这两个生理过程的有趣联系。(C)2015爱思唯尔有限公司版权所有。
Polyphenisms such as wing dimorphisms and caste determination are important in allowing animals to adapt to changing environments. The brown planthopper Nilaparvata lugens, one of the most serious insect agricultural pests, includes two wing forms, the long wing form (macropterous) and the short wing form (brachypterous). Long wings are specialized for migration, while short wings are found in individuals specialized for reproduction. While studying wing form polyphenism in the brown planthopper, we excised single wing pads from 4th instar nymphs in order to preserve transcriptional records to correlate with adult wing form. Surprisingly, we found that excision of one wing pad from a pair of the forewings changed the wing morph of the other wing after development to the adult, resulting in the short wing morph. Further experiments showed that not only excision or slicing of the wing pad, but also needle punctures in the abdomen all caused a significant increase in the proportion of nymphs developing into short winged adults. Thus wounding appears to cause a shift to short wing development. We then tested the transcriptional expression in N. lugens of the transcription factor FOXO, which has been shown to help mediate both wing polyphenism in brown planthoppers and wound healing in mice, after excision of the wing pad. Both NlFOXO and its downstream target Nl4EBP increased significantly after wing pad excision. These results indicate that FOXO mediates both wing development and wound healing in N. lugens, which results in an interesting linkage of these two physiological processes. (C) 2015 Elsevier Ltd. All rights reserved.