Highly temporal stable, wavelength-independent, and scalable field-of-view common-path quantitative phase microscope.
Highly temporal stable, wavelength-independent, and scalable field-of-view common-path quantitative phase microscope.
复制标题
DOI：
10.1117/1.jbo.25.11.116501
复制
发表时间：
2020-11
影响因子：
3.5
通讯作者：
Mehta DS
中科院分区：
文献类型：
--
作者：
Ahmad A;Dubey V;Butola A;Ahluwalia BS;Mehta DS
意义：高时间稳定性、波长无关性和可扩展视场(FOV)是定量位相显微镜(QPM)系统的主要要求。该系统的高时间稳定性提供了对生物细胞微小膜波动的准确测量，这可以作为疾病诊断的指标。目的：这项工作的主要目的是发展一种高时间稳定性的技术，能够准确地量化细胞的动力学，如人红细胞膜的波动。此外，该技术应该能够在多个波长获得可缩放的视场和分辨率，以使其适用于各种生物应用。方法：我们开发了一种单元素近公共路径、波长无关、可扩展的分辨率/视场QPM系统，以获得生物样品的时间稳定的全息图/干涉图。结果：在不使用隔振台的情况下，该系统具有较好的时间稳定性。在USAF分辨率图和聚苯乙烯小球(直径)上首次证明了该系统的能力。此外，该系统还利用彩色CCD摄像机实现了对具有可缩放分辨率的人红细胞(RBC)的单次拍摄、与波长无关的定量相位成像。还测量了健康人红细胞膜的波动，发现约为47纳米。结论：与光学系统相反，本系统为共光路准分子激光器的研制提供了一种节能、经济、简便的物光和参考光束产生方法。本系统为用户提供了在可缩放视场和分辨率下获取多波长定量相位图像的灵活性。
Significance: High temporal stability, wavelength independency, and scalable field of view (FOV) are the primary requirements of a quantitative phase microscopy (QPM) system. The high temporal stability of the system provides accurate measurement of minute membrane fluctuations of the biological cells that can be an indicator of disease diagnosis. Aim: The main aim of this work is to develop a high temporal stable technique that can accurately quantify the cell’s dynamics such as membrane fluctuations of human erythrocytes. Further, the technique should be capable of acquiring scalable FOV and resolution at multiple wavelengths to make it viable for various biological applications. Approach: We developed a single-element nearly common path, wavelength-independent, and scalable resolution/FOV QPM system to obtain temporally stable holograms/interferograms of the biological specimens. Results: With the proposed system, the temporal stability is obtained without using any vibration isolation table. The capability of the proposed system is first demonstrated on USAF resolution chart and polystyrene spheres ( diameter). Further, the system is implemented for single shot, wavelength-independent quantitative phase imaging of human red blood cells (RBCs) with scalable resolution using color CCD camera. The membrane fluctuation of healthy human RBCs is also measured and was found to be around 47 nm. Conclusions: Contrary to its optical counterparts, the present system offers an energy efficient, cost effective, and simple way of generating object and reference beam for the development of common-path QPM. The present system provides the flexibility to the user to acquire multi-wavelength quantitative phase images at scalable FOV and resolution.