Preparation and characterization of bacterial cellulose sponge with hierarchical pore structure as tissue engineering scaffold
Preparation and characterization of bacterial cellulose sponge with hierarchical pore structure as tissue engineering scaffold
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DOI：
10.1007/s10934-010-9364-6
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发表时间：
2011-04-01
影响因子：
2.6
通讯作者：
Wang, Jiehua
中科院分区：
文献类型：
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作者：
Gao, Chuan;Wan, Yizao;Wang, Jiehua
细菌纤维素(BC)被认为是一种有前途的、低成本的组织工程纳米支架材料。然而，BC的孔径不够大，不适合细胞的生长，限制了其作为组织工程支架的实际应用。本工作采用乳化冷冻干燥技术制备了新型多孔BC海绵。扫描电子显微镜(SEM)和压汞测孔仪的结果表明，所制备的BC海绵由纳米纤维组成，具有层次化的孔结构，由大孔(直径为20~1000微米)和纳米孔(直径约为4 nm)组成。BC海绵具有较高的比表面积(92.81+/-2.02 m(2)/g)和足够的孔隙率(90.42+/-0.24%)。此外，BC海绵的大小和形状可以通过使用适当的模具很容易地控制。我们还证实了BC海绵具有良好的细胞相容性，因为纤维滑膜来源的间充质干细胞(MSCs)可以在BC海绵的表面和内部良好地增殖，培养7d后最大种植距离可达150微米。
Bacterial cellulose (BC) is believed to be a promising and cost-efficient nano-scaffold for tissue engineering. However, the pore size of BC is not big enough for cell ingrowth, which restricts its practical usage as tissue engineering scaffold. In this work, novel porous BC sponges were obtained through emulsion freeze-drying technique. Results of scanning electron microsc.opy (SEM) and mercury intrusion porosimeter showed that the resulted BC sponges were composed of nanofibrills with hierarchical pore structure consisting of large pores (from 20 to similar to 1,000 mu m in diameter) and nano pores (down to similar to 4 nm in diameter). BC sponges possessed high surface area (92.81 +/- 2.02 m(2)/g) and sufficient porosity (90.42 +/- 0.24%). Additionally, the size and shape of BC sponges could be easily controlled by using appropriate molds. We also demonstrated that BC sponges had excellent cell compatibility as fibrous synovium-derived mesenchymal stem cells (MSCs) could proliferate well on and inside the BC sponges and the maximum ingrowth distance was 150 mu m after cultured for 7 days.