Antigen-binding diversity and idiotypic cross-reactions among hybridoma autoantibodies to DNA.
Antigen-binding diversity and idiotypic cross-reactions among hybridoma autoantibodies to DNA.
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杂交瘤 DNA 自身抗体之间的抗原结合多样性和独特型交叉反应。
DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
Robert S. Schwartz
中科院分区：
文献类型：
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作者：
Chester Andrzejewski;J. Rauch;Eileen M. Lafer;B. Stollar;Robert S. Schwartz
由来自未免疫的MRL/L小鼠与BALB/C浆细胞纤维瘤的脾细胞融合产生的克隆产生的杂交瘤抗DNA抗体的结合，以与核酸抗原的结合以及与抗IDiot型抗体的反应率。所有抗体结合到多种核酸和多核苷酸。这一发现表明，核酸抗原共有的表位可以解释狼疮血清中的某些血清学多样性。每种杂交瘤自身抗体与核酸抗原具有独特的反应性模式，但通过抗IDiotype抗体的分析发现了其中一些的交叉反应。这些交叉反应是由于自身抗体的抗原结合区域的相似性，因为抗IDiotype IG阻断了杂交瘤抗体DNA的结合。此外，多核苷酸抑制了白痴型抗IDiotype反应。在源自同一MRL/L小鼠的克隆产生的杂交瘤抗DNA抗体中，还发现了交叉反应的白痴，而且发现了来自不同MRL/L小鼠的克隆产生的抗体。后一个发现表明，生殖系基因的家族可能会针对DNA编码一些自身抗体。
Hybridoma anti-DNA antibodies produced by clones derived from fusions of spleen cells from unimmunized MRL/l mice with BALB/c plasmacytomas were examined for their binding to nucleic acid antigens and for their reactivities with anti-idiotype antibodies. All the antibodies bound to a variety of nucleic acids and polynucleotides. This finding suggests that epitopes shared by nucleic acid antigens can account for some of the serologic diversity in lupus serum. Each hybridoma autoantibody had a unique pattern of reactivity with nuclei acid antigens, yet cross-reactions among some of them were found by analyses with anti-idiotype antibodies. These cross-reactions were due to similarities in the antigen-binding regions of the autoantibodies because anti-idiotype Ig blocked the binding of the hybridoma antibody DNA; moreover, polynucleotides inhibited the idiotype anti-idiotype reaction. Cross-reactive idiotypes were found not only among hybridoma anti-DNA antibodies produced by clones derived from the same MRL/l mice but also among antibodies produced by clones derived from different MRL/l mice. The latter finding suggests that families of germ line genes may encode some autoantibodies against DNA.