Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis
Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis
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DOI：
10.1046/j.1365-2672.2002.01502.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
König, H
中科院分区：
文献类型：
--
作者：
Wenzel, M;Schönig, I;König, H
目的：为证实狭颈动白蚁体内存在纤维素分解菌，采用好氧培养法从狭颈动白蚁肠道中分离到119株纤维素分解菌。颈窄菌，它们被分配给23组需氧、兼性厌氧或微需氧纤维素分解细菌。利用16 S rDNA限制性内切酶图谱和部分16 S rDNA序列分析以及数值分类学方法对分离株进行了分类。放线菌分支中的革兰氏阳性菌属于放线菌目（Actinomycetales），包括纤维单胞菌属（CellulomonasOerskovia）、微杆菌属（Microbacterium）和库克菌属（Kocuria）。来自芽孢杆菌目的革兰氏阳性菌属于芽孢杆菌属、短芽孢杆菌属和类芽孢杆菌属。与来自α-变形菌门的Afipia属、土壤杆菌属/根瘤菌属、布鲁氏菌属/苍白杆菌属、假单胞菌属和鞘氨醇单胞菌属/发酵单胞菌属相关的分离物以及来自“嗜热杆菌科”的类螺旋体菌属代表革兰氏阴性菌。某些分离株的细胞滴度高达10（7）个纤维素分解菌/ml，表明除了纤维素分解鞭毛和白蚁自身的纤维素酶外，它们还可能在白蚁肠道的纤维素消化中发挥作用。细菌对白蚁肠道中纤维素降解的影响一直是一个有争议的问题。本研究调查了白蚁肠道中的好氧和兼性厌氧纤维素分解菌。
Aims: To demonstrate the occurrence of cellulolytic bacteria in the termite Zootermopsis angusticollis.Methods and Results: Applying aerobic cultivation conditions the isolated 119 cellulolytic strains from the gut of Z. angusticollis, which were assigned to 23 groups of aerobic, facultatively anaerobic or microaerophilic cellulolytic bacteria. 16S rDNA restriction fragment pattern and partial 16S rDNA sequence analysis, as well as numerical taxonomy, were used for the assignment of the isolates. The Gram-positive bacteria of the actinomycetes branch could be assigned to the order Actinomycetales including the genera Cellulomonas/Oerskovia, Microbacterium and Kocuria. The Gram-positive bacteria from the order Bacillales belonged to the genera Bacillus, Brevibacillus and Paenibacillus. Isolates related to the genera Afipia, Agrobacterium/Rhizobium, Brucella/Ochrobactrum, Pseudomonas and Sphingomonas/Zymomonas from the alpha-proteobacteria and Spirosoma-like from the "Flexibacteriaceae" represented the Gram-negative bacteria.Conclusions: A cell titre of up to 10(7) cellulolytic bacteria per ml, determined for some isolates, indicated that they may play a role in cellulose digestion in the termite gut in addition to the cellulolytic flagellates and termite's own cellulases.Significance and Impact of the Study: The impact of bacteria on cellulose degradation in the termite gut has always been a matter of debate. In the present survey we investigated the aerobic and facultatively anaerobic cellulolytic bacteria in the termite gut.