Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid
Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid
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DOI：
10.1111/rda.13417
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发表时间：
2019-03
影响因子：
1.7
通讯作者：
Nhien Thi Nguyen;M. Hirata;F. Tanihara;T. Hirano;Q. Le;M. Nii;T. Otoi
中科院分区：
文献类型：
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作者：
Nhien Thi Nguyen;M. Hirata;F. Tanihara;T. Hirano;Q. Le;M. Nii;T. Otoi
本研究旨在探讨100%胎牛血清（FBS）和100%猪卵泡液（pFF）作为储存介质对25°C储存24小时的猪受精卵发育能力的影响。此外，我们还评估了绿原酸（CGA）在存储介质中的附加效应。当体外产生的受精卵在含有补充有 1 mg/ml 牛血清白蛋白 (BSA)、100% FBS 或 100% pFF 的组织培养基 (TCM) 199 的试管中于 25°C 保存 24 小时时，100% FBS 中的囊胚形成率显着高于含有 BSA 的 TCM 199。 对在 25°C 储存 24 小时的受精卵发育中的 FBS 进行了 100% 评估，与其他浓度的 CGA 相比，用 50 µM CGA 储存的受精卵发育为囊胚的数量更多。当对储存在补充有 50 µM CGA 的 100% FBS 中的受精卵的囊胚形成日期和质量进行研究时，储存组囊胚形成的最高比率出现比未储存对照组晚 1 天。然而，与非保存组相比，保存组中观察到具有凋亡细胞核的囊胚比例更高。总之，100%的FBS可用于猪受精卵的短期储存介质。在储存介质中添加50 µM CGA可提高储存后受精卵的囊胚形成率，但储存受精卵的胚胎质量仍有待提高。
The current study was conducted to investigate the effects of 100% foetal bovine serum (FBS) and 100% porcine follicular fluid (pFF) as a storage medium on the developmental competence of porcine zygotes stored at 25°C for 24 hr. Moreover, we evaluated the additive effects of chlorogenic acid (CGA) in the storage medium. When in vitro-produced zygotes were stored at 25°C for 24 hr in tubes containing either tissue culture medium (TCM) 199 supplemented with 1 mg/ml bovine serum albumin (BSA), 100% of FBS or 100% of pFF, the rate of blastocyst formation was significantly higher in 100% of FBS than in BSA-containing TCM 199. When the effects of CGA supplementation in 100% of FBS on the development of zygotes stored at 25°C for 24 hr was evaluated, more zygotes stored with 50 µM CGA developed to blastocysts compared with the other concentrations of CGA. When the formation date and quality of blastocysts derived from zygotes stored in 100% of FBS supplemented with 50 µM CGA were investigated, the highest ratio of blastocysts formation in the storage group appeared 1 day later than in the non-stored control group. However, a higher proportion of blastocysts with apoptotic nuclei was observed in the stored group as compared to the non-stored group. In conclusion, 100% of FBS is available for a short storage medium of porcine zygotes. The supplementation of 50 µM CGA into the storage medium improves the rates of blastocyst formation of zygotes after storage, but the quality of embryos from the stored zygotes remains to be improved.