Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog
Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog
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DOI：
10.1126/science.1138527
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发表时间：
2007-03-02
期刊：
影响因子：
56.9
通讯作者：
Mullins, R. Dyche
中科院分区：
文献类型：
--
作者：
Garner, Ethan C.;Campbell, Christopher S.;Mullins, R. Dyche
多个不相关的聚合物系统已经进化成在分裂时在子细胞之间划分DNA分子。为了更好地理解聚合物驱动的DNA分离，我们从纯化的组分中重构了R1质粒的三组分分离系统。我们发现，PARR/PARC复合体可以通过结合PARM细丝的末端，抑制动态不稳定性，并充当棘轮，允许加入新的单体并骑在延长的细丝末端，从而构建一个简单的双极纺锤体。在稳态条件下，未附着的Parm细丝的动态不稳定性提供了驱动DNA分离所需的能量。
Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.