The location of iron and zinc in grain of conventional and biofortified lines of sorghum
The location of iron and zinc in grain of conventional and biofortified lines of sorghum
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DOI：
10.1016/j.jcs.2022.103531
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发表时间：
2022-09-01
影响因子：
3.8
通讯作者：
Shewry, Peter R.
中科院分区：
文献类型：
--
作者：
Gaddameedi, Anil;Sheraz, Sadia;Shewry, Peter R.
在非洲和印度部分地区，高粱是膳食铁和锌的重要来源，但需要提高其浓度以满足膳食需求。生物强化高粱品系（Parbhani Shakti）籽粒铁和锌含量高于对照品系（M35-1）。通过组织化学染色和NanoSIMS在细胞水平上的分析表明，这两种矿物质都集中在胚胎的糊粉层和鳞片中，锌也集中在胚胎轴中。然而，NanoSIMS显示，在亚糊粉和淀粉胚乳细胞中也存在Fe-56（+）和Zn-64（+）的“热点”。这些热点大部分还含有(PO+)-P-31-O-16，表明铁和锌以植酸盐的形式存在，就像在糊粉细胞和鳞片细胞中一样。在这些细胞的蛋白质基质中也观察到低浓度的Fe-56（+）和Zn-64（+）。
Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that "hot spots" of Fe-56(+) and Zn-64(+) were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained (PO+)-P-31-O-16 indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of Fe-56(+) and Zn-64(+) were also observed in the protein matrix of these cells.