Effects of matriconditioning on onion seed germination, seedling emergence and associated physical and metabolic events
Effects of matriconditioning on onion seed germination, seedling emergence and associated physical and metabolic events
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DOI：
10.1023/b:grow.0000007509.94430.eb
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发表时间：
2003-11-01
影响因子：
4.2
通讯作者：
Kepczynski, J
中科院分区：
文献类型：
--
作者：
Kepczynska, E;Piekna-Grochala, J;Kepczynski, J
采用Micro-Cel E对洋葱进行生理催芽处理。CV.研究了车尔尼亚科夫斯卡种子的品质。试验了种子、载体、水分和引发时间的几种配比。在大多数测试温度下提高洋葱种子发芽的最有效的处理是在15 ℃下在光照下以2 g种子：1 g Micro-Cel：3 g水的比例引发5天。Matriconditioning大大提高了发芽率和出苗率，幼苗鲜重和干重，减少电解质渗漏相比，未经处理的种子，这种有益的效果是特别明显的在次优温度。Matriconditioning提高了老化种子的发芽率，效果更显着，在更老的种子。在无前体物质1-氨基环丙烷-1-羧酸（ACC）存在的情况下，引发和未引发种子的乙烯产量没有显著差异，但在吸胀过程中ACC的存在引起乙烯产量的增加;与未处理的种子相比，在洋葱基质处理的种子中体内ACC氧化酶的活性增强，表明内源ACC水平是乙烯产生的限制因子。同样，从处理过的种子中分离的ACC氧化酶的活性高于未处理的种子。与未引发的种子相比，在引发的空气干燥种子中观察到更高的内切β-甘露聚糖酶和总脱氢酶活性。
The effect of matriconditioning, the physiological presowing seed technique, using Micro-Cel E on Allium cepa L. cv. Czerniakowska seed quality was studied. Several ratios of seeds, carrier, water and time of priming were tested. The most effective treatment for improving onion seed germination at most tested temperatures was priming to a ratio of 2 g seed : 1 g Micro-Cel : 3 g water for 5 days in light at 15 degreesC. Matriconditioning greatly improved the germination and emergence percentage, seedling fresh and dry weight and reduced electrolyte leakage compared to that of untreated seeds; this beneficial effect was especially evident at suboptimal temperatures. Matriconditioning improved the germinability of aged seeds, the effect being more pronounced in the more aged seeds. No significant differences in ethylene production by primed and non-primed seeds were observed in the absence of its precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), but its presence during imbibition caused an increase in ethylene production; an enhanced activity of in vivo ACC oxidase in Allium cepa matriconditioned seeds in comparison to untreated seeds, indicates that the endogenous level of ACC is a limiting factor of ethylene production. Likewise, the activity of ACC oxidase isolated from matriconditioned seeds was higher than that from untreated seeds. Higher endo-beta-mannanase and total dehydrogenase activities were observed in primed air-dried seeds in comparing to non-primed seeds.