In Situ Growth Kinetics of Hydroxyapatite on Electrospun Poly(DL-lactide) Fibers with Gelatin Grafted
In Situ Growth Kinetics of Hydroxyapatite on Electrospun Poly(DL-lactide) Fibers with Gelatin Grafted
复制标题
羟基磷灰石在明胶接枝电纺聚（DL-丙交酯）纤维上的原位生长动力学
DOI：
10.1021/cg800641s
复制
发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Weng, Jie
中科院分区：
文献类型：
--
作者：
Cui, Wenguo;Li, Xiaohong;Weng, Jie
羟基磷灰石（HA）和聚乳酸（PDLLA）的纤维纳米复合材料的发展为组织工程支架提供了巨大的潜力。已经尝试解决HA微晶在纤维垫中聚集的问题，以提高机械性能和生物活性。本研究提出了一种新的方法，使用静电纺丝PDLLA纳米纤维与表面改性作为复合材料制造的诱导位点。通过优化的氨解工艺在静电纺丝纤维上生成游离氨基，并通过戊二醛偶联将明胶进一步接枝到氨解纤维上。在氨解和明胶接枝过程中，氨基和明胶的量可以用定量方程作为氨解时间的函数来建模。确定了非化学计量比纳米结构HA的形成和在静电纺丝纤维上的良好分散。实验证明明胶可以控制纤维膜上晶体的成核和生长。
Development of fibrous nanocomposites of hydroxyapatite (HA) and poly(dl-lactide) (PDLLA) offers great potential as tissue engineering scaffolds. Attempts have been made to solve problematic issues of the agglomeration of HA crystallites in fibrous mats to enhance the mechanical property and bioactivity. A novel method was introduced in the present study to use electrospun PDLLA nanofibers with surface modifications as the induction sites for composite fabrication. Free amino groups were created on electrospun fibers through an optimized aminolysis process, and gelatin was further grafted on the aminolyzed fibers through glutaraldehyde coupling. In the aminolysis and gelatin grafting process, the amount of amino groups and gelatin could be modeled with quantitative equations as a function of the aminolysis time. The formation of nonstoichiometric nanostructured HA and good dispersion on electrospun fibers were determined. Gelatin was demonstrated to control the nucleation and growth of crystals on fibrous...