Enhanced catalytic activity of gold nanoparticle-carbon nanotube hybrids for influenza virus detection
Enhanced catalytic activity of gold nanoparticle-carbon nanotube hybrids for influenza virus detection
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DOI：
10.1016/j.bios.2016.05.050
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发表时间：
2016-11-15
影响因子：
12.6
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
--
作者：
Ahmed, Syed Rahin;Kim, Jeonghyo;Park, Enoch Y.
多功能纳米杂化物为广泛的催化和生物技术应用创造了新的宝贵机会。在这里，我们提出了一种相对简单的方法来生产由金纳米粒子（Au NP）和碳纳米管（CNT）组成的纳米杂化物，不需要对CNT进行酸性预处理。透射电子显微镜 (TEM) 图像和紫外可见 (UV-vis) 光谱显示 Au 纳米颗粒键合到 CNT 表面。表面增强拉曼散射 (SERS) 显示，Au-CNT 纳米杂化物的信号比原始 CNT 的信号更强。 Au-CNT纳米杂化物在H2O2氧化3,3',5,5'-四甲基联苯胺(TMB)时表现出催化活性，并在水溶液中呈现出独特的蓝色。由于这些 Au-CNT 纳米杂化物具有增强的过氧化物酶样活性，因此选择它们作为甲型流感病毒 (H3N2) 高灵敏度比色测试的一部分。当测试系统（特异性抗体缀合的 Au CNT 纳米杂化物-TMB-H2O2）中存在甲型流感病毒 (H3N2) 时，会出现深蓝色，其光密度取决于病毒浓度 (10-50,000 PFU/ml)。该方法的检测限为3.4 PFU/ml，比传统ELISA（1312 PFU/ml）低385倍。该测试的灵敏度也比商业免疫层析试剂盒高 500 倍。本文报道的纳米杂化制剂和比色检测方法可以很容易地适应具有酶模拟特性的其他纳米杂化结构，以在催化和纳米生物技术中得到更广泛的应用。 (C) 2016 Elsevier B.V. 保留所有权利。
Multifunctional nanohybrids have created new and valuable opportunities for a wide range of catalysis and biotechnology applications. Here, we present a relatively simple method for producing nanohybrids composed of gold nanoparticles (Au NPs) and carbon nanotubes (CNTs) that does not require an acidic pretreatment of the CNTs. Transmission electron microscopy (TEM) images and ultraviolet-visible (UV-vis) spectra revealed that Au NPs bonded to the CNT surface. Surface-enhanced Raman scattering (SERS) revealed a stronger signal from Au-CNT nanohybrids than from pristine CNTs. The Au-CNT nanohybrids showed catalytic activity in the oxidation of 3, 3', 5, 5'-tetramethyl-benzidine (TMB) by H2O2 and developed a unique blue colour in aqueous solution. Because of the enhanced peroxidase-like activity of these Au-CNT nanohybrids, they were selected for use as part of a highly sensitive colorimetric test for influenza virus A (H3N2). In the presence of influenza A virus (H3N2) in the test system (specific antibody-conjugated Au CNT nanohybrids-TMB-H2O2), a deep blue colour developed, the optical density of which was dependent on the virus concentration (10-50,000 PFU/ml). The limit of detection of this proposed method was 3.4 PFU/ml, a limit 385 times lower than that of conventional ELISA (1312 PFU/ml). The sensitivity of this test was also 500 times greater than that of commercial immunochromatography kits. The nanohybrid preparation and colorimetric detection methods reported herein may be easily adapted to other nanohybrid structures with enzyme mimetic properties for broader applications in catalysis and nanobiotechnology. (C) 2016 Elsevier B.V. All rights reserved.