Spectrum-Effect Relationships Between Chemical Fingerprints and Antibacterial Effects of Lonicerae Japonicae Flos and Lonicerae Flos Base on UPLC and Microcalorimetry.
Spectrum-Effect Relationships Between Chemical Fingerprints and Antibacterial Effects of Lonicerae Japonicae Flos and Lonicerae Flos Base on UPLC and Microcalorimetry.
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DOI：
10.3389/fphar.2016.00012
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发表时间：
2016
影响因子：
5.6
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Shi Z;Liu Z;Liu C;Wu M;Su H;Ma X;Zang Y;Wang J;Zhao Y;Xiao X
中药材金银花(LJF，金银花)和金银花(Lf，山银花)是指忍冬科五种植物的花蕾。直到2000年，所有这些都被正式列入《中国药典》的单一药材--金银花。然而，关于行政法规中LJF和LF的分离与合并，近年来学术界存在较多争议。到目前为止，评价这些药物之间的生物活性和化学性质之间的关系的工作还很少完成。采用微量热法和超高效电化学法，结合主成分分析(PCA)、层次聚类分析(HCA)和典型相关分析(CCA)研究了LJF和LF的化学成分与抑菌效果之间的关系。采用多元统计分析方法，根据LJF和LF的化学指纹图谱初步区分LJF和LF，将两种中草药的抗菌作用分为两类。这一结果支持了药典委员会对LJF和LF的拆分。然而，黄褐毛忍冬的样品被证明是一个中间物种，其抗菌效果与LJF相似。CCA分析结果表明，绿原酸和3，4-二咖啡酰奎宁酸是产生抑菌作用的主要成分。此外，3，4-二咖啡酰奎宁酸可作为LJF和LF质量控制的新标志成分。
The traditional Chinese medicines Lonicerae Japonicae Flos (LJF, Jinyinhua in Chinese) and Lonicerae Flos (LF, Shanyinhua in Chinese) refer to the flower buds of five plants belonging to the Caprifoliaceae family. Until 2000, all of these were officially listed as a single item, LJF (Jinyinhua), in the Chinese Pharmacopoeia. However, there have recently been many academic controversies concerning the separation and combination of LJF and LF in administrative regulation. Till now there has been little work completed evaluating the relationships between biological activity and chemical properties among these drugs. Microcalorimetry and UPLC were used along with principal component analysis (PCA), hierarchical cluster analysis (HCA), and canonical correlation analysis (CCA) to investigate the relationships between the chemical ingredients and the antibacterial effects of LJF and LF. Using multivariate statistical analysis, LJF and LF could be initially separated according to their chemical fingerprints, and the antibacterial effects of the two herbal drugs were divided into two clusters. This result supports the disaggregation of LJF and LF by the Pharmacopoeia Committee. However, the sample of Lonicera fulvotomentosa Hsu et S. C. Cheng turned out to be an intermediate species, with similar antibacterial efficacy as LJF. The results of CCA indicated that chlorogenic acid and 3,4-Dicaffeoylquinic acid were the major components generating antibacterial effects. Furthermore, 3,4-Dicaffeoylquinic acid could be used as a new marker ingredient for quality control of LJF and LF.