REGULATED TRANSCRIPTION OF THE MAIZE BRONZE-2 PROMOTER IN ELECTROPORATED PROTOPLASTS REQUIRES THE C1 AND R GENE-PRODUCTS
REGULATED TRANSCRIPTION OF THE MAIZE BRONZE-2 PROMOTER IN ELECTROPORATED PROTOPLASTS REQUIRES THE C1 AND R GENE-PRODUCTS
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DOI：
10.1007/bf00265434
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发表时间：
1992-06-01
期刊：
影响因子：
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通讯作者：
WALBOT, V
中科院分区：
文献类型：
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作者：
BODEAU, JP;WALBOT, V
推定的玉米转录因子基因R和C1是具有来自Bz 1和A1基因的启动子的报告基因表达所需的，所述Bz 1和A1基因编码玉米中花青素苷生物合成所需的酶。BZ 2是另一个花青素生物合成基因，我们表明，从BZ 2启动子的报告基因的表达也需要R和C1时，融合构建体被引入到玉米籽粒通过粒子枪轰击。当从不合成花青素的悬浮细胞系电穿孔到玉米原生质体中时，具有Bz 2、Bz 1和A1启动子的报告基因仅在R和C1表达质粒共电穿孔时表达。电穿孔的R和C1表达质粒也诱导花色素苷合成所需的内源基因，导致粉红色的原生质体在24小时内。RNA酶保护分析表明，从内源性Bz 1和Bz 2基因的mRNA的积累绝对需要引入R和C1。在时程实验中，Bz 2启动子被激活之前有3-6小时的延迟，支持R和C1编码蛋白在转录控制中的拟议作用。过量的R相对于C1抑制A1，Bz 1和Bz 2启动子的表达，表明R和C1蛋白之间的相互作用。
The putative maize transcription factor genes R and C1 are required for expression of reporter genes with promoters from the Bz1 and A1 genes, which encode enzymes required for anthocyanin biosynthesis in maize. Bz2 is another anthocyanin biosynthetic gene; we show that expression of a reporter gene from the Bz2 promoter also requires R and C1 when the fusion construct is introduced into maize kernels by particle gun bombardment. When electroporated into maize protoplasts from a suspension cell line not synthesizing anthocyanins, reporter genes with Bz2, Bz1, and A1 promoters are expressed only when both R and C1 expression plasmids are co-electroporated. Electroporation of R and C1 expression plasmids also induces the endogenous genes required for anthocyanin synthesis, resulting in pink protoplasts within 24 h. RNase protection analysis demonstrates that accumulation of mRNA from the endogenous Bz1 and Bz2 genes absolutely requires introduced R and C1. In time-course experiments there is a delay of 3-6 h before the Bz2 promoter is activated, supporting the proposed role for R- and C1-encoded proteins in transcriptional control. An excess of R relative to C1 suppresses expression of A1, Bz1, and Bz2 promoters, suggesting an interaction between the R and C1 proteins.