Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex.
Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex.
复制标题
DOI：
10.1111/febs.14301
复制
发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Mackay JP
中科院分区：
文献类型：
--
作者：
Torrado M;Low JKK;Silva APG;Schmidberger JW;Sana M;Sharifi Tabar M;Isilak ME;Winning CS;Kwong C;Bedward MJ;Sperlazza MJ;Williams DC Jr;Shepherd NE;Mackay JP
核小体重塑和脱乙酰酶（NuRD）复合体对于复杂动物的发育是必不可少的。NuRD在调节基因表达和修复受损DNA中起作用。该复合物包含至少六种蛋白质，当从细胞提取物中纯化该复合物时，常规鉴定出每种蛋白质的两种或更多种旁系同源物。为了了解NuRD的结构和功能，需要直接亚基相互作用的图谱。许多已发表的研究试图定义直接的亚基间连接。我们认为，在许多这样的研究报告的结论实际上是模糊的几个原因之一。首先，许多NuRD亚基在细菌中的表达不太可能导致折叠的活性蛋白。其次，在含有内源性NuRD复合物的细胞中进行的相互作用研究可通过内源性组分桥接靶蛋白而导致假阳性。结合现有的信息NuRD结构的协议，旨在尽量减少假阳性，我们报告了一个保守的和强大的相互作用图NuRD复杂。我们还提出了一个复杂的3D模型，汇集了复杂的现有数据。本文讨论的问题和策略也适用于广泛的多亚基复合物的分析。NuRD复合物对复杂动物的基因调控至关重要。为了理解它的结构，需要直接亚基相互作用的图谱。我们评估了该领域已发表数据的可靠性，并通过实验绘制了相互作用图，以开发NuRD复合物的高置信度相互作用图。
The nucleosome remodelling and deacetylase (NuRD) complex is essential for the development of complex animals. NuRD has roles in regulating gene expression and repairing damaged DNA. The complex comprises at least six proteins with two or more paralogues of each protein routinely identified when the complex is purified from cell extracts. To understand the structure and function of NuRD, a map of direct subunit interactions is needed. Dozens of published studies have attempted to define direct inter-subunit connectivities. We propose that conclusions reported in many such studies are in fact ambiguous for one of several reasons. First, the expression of many NuRD subunits in bacteria is unlikely to lead to folded, active protein. Second, interaction studies carried out in cells that contain endogenous NuRD complex can lead to false positives through bridging of target proteins by endogenous components. Combining existing information on NuRD structure with a protocol designed to minimize false positives, we report a conservative and robust interaction map for the NuRD complex. We also suggest a 3D model of the complex that brings together the existing data on the complex. The issues and strategies discussed herein are also applicable to the analysis of a wide range of multi-subunit complexes. The NuRD complex is essential for gene regulation in complex animals. To understand its structure, a map of direct subunit interactions is needed. We assessed the reliability of published data on this area and experimentally mapped interactions to develop a high-confidence interaction map of the NuRD complex.
登录
查看更多内容
影响因子：
4.8
作者：
Low, Jason K. K.;Webb, Sarah R.;Mackay, Joel P.
通讯作者：
Mackay, Joel P.
DOI：
10.1073/pnas.1015341108
发表时间：
2011-05-03
影响因子：
11.1
作者：
Gnanapragasam, Merlin Nithya;Scarsdale, J. Neel;Williams, David C., Jr.
通讯作者：
Williams, David C., Jr.
影响因子：
3.3
作者：
Kon, C;Cadigan, KM;Nusse, R
通讯作者：
Nusse, R
DOI：
10.1074/jbc.m110.195842
发表时间：
2011-01-14
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lejon S;Thong SY;Murthy A;AlQarni S;Murzina NV;Blobel GA;Laue ED;Mackay JP
通讯作者：
Mackay JP
影响因子：
4.8
作者：
Brackertz, M;Boeke, J;Renkawitz, R
通讯作者：
Renkawitz, R