TBP binding to the TATA box induces a specific downstream unwinding site that is targeted by pluramycin.
TBP binding to the TATA box induces a specific downstream unwinding site that is targeted by pluramycin.
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TBP 与 TATA 盒的结合诱导了普拉霉素靶向的特定下游解旋位点。
DOI：
10.1016/1074-5521(95)90263-5
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发表时间：
1995
影响因子：
--
通讯作者：
Hurley,LH
中科院分区：
文献类型：
--
作者：
Sun,D;Hurley,LH
背景：TATA结合蛋白(TUP)是位于TATA序列下游的一种蛋白，它通过与L核糖核酸(TFIIL1)NA结合而产生明显的缺失成分。多个Cln加合物是连续的。对指导生产Ofuna链断裂产物RNAtr检测到的起始具有重要意义。说明：加热时，这是一个位于城市中心下游的地方。将多发性霉素与Cukar-Tul‘-DNA复合体中的TATA序列(TATA盒)孵育，有助于捕获特定的耳原虫。用插层法制备了TUP络合物的晶体结构。凝胶高贵位移和与TATA盒中的DN寡核苷酸的循环分析表明，多核苷酸与TATA盒区域近二重SYN-3‘侧的Jed J蛋白的结合相当明显地与TbL’-DNA复合体具有对称的相互作用。11NA。目前尚不清楚这种不对称效应是如何涉及的。结论：我们认为Dppar-三元络合物所产生的(TI-IP-11)Na-多元络合物所能激发的是TBP在其中的J‘特异型结合信息节点。我们用多环素检测了IINA在Tul‘-Lina复合体中的状态和多环素m，如DNA中的补偿性损伤，说明了1)NA可及性的另一种气味小分子探针的稳定性得到了改善。很复杂。WC还提出了三元cnplrx结果的J模型：TUI‘与TATA盒的重叠有助于解释所观察到的在J定义的位置与TATA盒即刻结合的多维霉素TUP 1-Jatlon的非同步cf&ct。
Background: The TATA-binding protein (TUP) is one ot downstream of the TATA sequence through an apparent thr nlajor components of the hunlan TFIIL1 niultiprotein transient un\vinding of the L) NA. Pluranlycln adducts are conlplc\-. It i\imporwlt in directing the initiation of detected by the production ofUNA strand breakage prod-RNA tr. ulscription ‘It a site innnediatcly downstream of ucts upon heating. Incubation of plurarnycin with the the TATA sequcncc (TATA box) found in n~ ly cukar- TUl’-DNA complex facilitates the trapping of the specific)-otic pronloters. The crystal structure ofTUP conlplexcd conlplex by u~ tercalation. Gel nnobility shift and circularwith dn oligonucleotide contannng the TATA box izntion assays reveal that the binding of pluralnycnl on the 1reveJed J protcin with an approximate two-fold syn- 3’-side of the TATA box region considerably Ttnbihze\the mctry XX hich Clpparently has symmetrical interaction\with TBl’-DNA complex. 11NA. It is not kno\\rn how an asymmetric effect involv- Conclusions: We propose that the TI-IP-11) NA-pluralrl~ cin ing do\\nstl-earn ‘lctivation can bv produced by an dppar- ternary complex is J ‘specific’binding inode in which TBP eiit c)-ninietric complex. We tet out to exaniine the state and plurCwiycin m, lke compensatory nltcrations in DNA, of IINA in thr TUl’-LINA complex using pluramycln, J accounting for the improved stability of the terndry smell molccula~-\veight probe of 1) NA accessibility. complex. WC also propose J model of the ternary cornplrx Results: Hillding of TUI’ to the TATA box facilitates that explains the observed asynnnetric cf&ct of TUP 1ntercJatlon of pluramvcin at J defined site imniediately binding to the TATA box.