Sexual dimorphism and the effect of wild introgressions on recombination in cassava (Manihot esculenta Crantz) breeding germplasm.
Sexual dimorphism and the effect of wild introgressions on recombination in cassava (Manihot esculenta Crantz) breeding germplasm.
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DOI：
10.1093/g3journal/jkab372
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发表时间：
2022-01-04
期刊：
影响因子：
--
通讯作者：
Jannink JL
中科院分区：
文献类型：
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作者：
Chan AW;Villwock SS;Williams AL;Jannink JL
减数分裂在减数分裂、进化和繁殖中具有重要的功能。交叉的频率和分布决定了新等位基因组合的产生，并且可以在物种和性别之间变化。在这里，我们研究重组景观在18条染色体的木薯（木薯）相对于男性和女性减数分裂和已知的基因渗入野生相对木薯glaziovii。我们使用SHAPEIT 2和duoHMM从基因分型测序数据和来自国际热带农业研究所木薯育种种质的经验证的多代系谱中推断出交叉，该种质由7020个信息性减数分裂组成。然后，我们构建了新的遗传图谱，并将其与国际木薯遗传图谱联盟先前构建的现有图谱进行了比较。我们观察到女性的重组率高于男性，而M的重组率较低。在染色体1和4上的glaziovii渐渗片段，在染色体4渐渗的情况下，沿着染色体的整个长度具有受抑制的重组沿着。最后，我们讨论了我们观察到的异交叉和交叉抑制的假设机制，并讨论了植物育种的更广泛的意义。
Recombination has essential functions in meiosis, evolution, and breeding. The frequency and distribution of crossovers dictate the generation of new allele combinations and can vary across species and between sexes. Here, we examine recombination landscapes across the 18 chromosomes of cassava (Manihot esculenta Crantz) with respect to male and female meioses and known introgressions from the wild relative Manihot glaziovii. We used SHAPEIT2 and duoHMM to infer crossovers from genotyping-by-sequencing data and a validated multigenerational pedigree from the International Institute of Tropical Agriculture cassava breeding germplasm consisting of 7020 informative meioses. We then constructed new genetic maps and compared them to an existing map previously constructed by the International Cassava Genetic Map Consortium. We observed higher recombination rates in females compared to males, and lower recombination rates in M. glaziovii introgression segments on chromosomes 1 and 4, with suppressed recombination along the entire length of the chromosome in the case of the chromosome 4 introgression. Finally, we discuss hypothesized mechanisms underlying our observations of heterochiasmy and crossover suppression and discuss the broader implications for plant breeding.