BCG vaccine powder-laden and dissolvable microneedle arrays for lesion-free vaccination.
BCG vaccine powder-laden and dissolvable microneedle arrays for lesion-free vaccination.
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DOI：
10.1016/j.jconrel.2017.03.397
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发表时间：
2017-06-10
期刊：
影响因子：
--
通讯作者：
Wu MX
中科院分区：
文献类型：
--
作者：
Chen F;Yan Q;Yu Y;Wu MX
Live attenuated Bacille Calmette-Guerin (BCG) bacillusis the only licensed vaccine for tuberculosis prevention worldwide to date. It must be delivered intradeemally to be effective, which causes severe skin inflammation and sometimes, permanent scars. To minimize the side effects, we developed a novel microneedle array (MNA) that could deliver live attenuated freeze-dried BCG powder into the epidermis in a painless, lesion-free, and self-applicable fashion. The MNA was fabricated with biocompatible and dissolvable hyaluronic acid with a deep cave formed in the basal portion of each microneedle, into which BCG powder could be packaged directly. Viability of BCG vaccine packaged in the caves and the mechanical strength of the powder-laden MNA did not alter significantly before and after more than two months of storage at room temperature. Following insertion of the MNA into the skin, the individual microneedle shafts melted away by interstitial fluid from the epidermis and upper dermis, exposing the powder to epidermal tissues. The powder sucked interstitial fluid, dissolved slowly, and diffused into the epidermis in a day against the interstitial fluid influx. Vaccination with BCG-MNA caused no overt skin irritation, in marked contrast to intradermal vaccination that provoked severe inflammation and bruise.While causing little skin irritation, vaccination efficacy of BCG-MNAs was comparable to that of intradermal immunization whether it was evaluated by humoral or cellular immunity. This powder-laden and dissolvable MNA represents a novel technology to sufficiently deliver live attenuated vaccine powders into the skin.
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影响因子：
105.7
作者：
Hawkridge, Anthony;Hatherill, Mark;Little, Francesca;Goetz, Margaret Ann;Barker, Lew;Mahomed, Hassan;Sadoff, Jerald;Hanekom, Willem;Geiter, Larry;Hussey, Greg
通讯作者：
Hussey, Greg
DOI：
10.1016/j.jconrel.2014.08.006
发表时间：
2014-10-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Chen X;Kositratna G;Zhou C;Manstein D;Wu MX
通讯作者：
Wu MX
影响因子：
30.3
作者：
Achkar JM;Casadevall A
通讯作者：
Casadevall A
影响因子：
7.3
作者：
Maria Garcia-Cuesta, Eva;Lopez-Cobo, Sheila;Vales-Gomez, Mar
通讯作者：
Vales-Gomez, Mar
影响因子：
3.2
作者：
Garcia-Pelayo, M. Carmen;Bachy, Veronique S.;Hogarth, Philip J.
通讯作者：
Hogarth, Philip J.