The fission yeast bromodomain protein Bdf2 is required for the growth of cells with circular chromosomes
The fission yeast bromodomain protein Bdf2 is required for the growth of cells with circular chromosomes
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DOI：
10.1093/bbb/zbab215
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发表时间：
2022-01-24
影响因子：
1.6
通讯作者：
Ueno，Masaru
中科院分区：
文献类型：
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作者：
Yasuda，Misaki;Habib，Ahmed G. K.;Ueno，Masaru
在间叶起源的肿瘤中经常观察到环状染色体。在裂殖酵母裂殖酵母中，pot 1+的缺失导致端粒的快速丢失，而产生的幸存者具有环状染色体。裂殖酵母有2个布罗莫结构域和末端外（BET）蛋白，Bdf 1和Bdf 2;两者都是维持乙酰化组蛋白所必需的。在这里，我们发现bdf 2（而不是bdf 1）对pot 1具有合成致死性。我们还获得了一个温度敏感的bdf 2-t突变体，它能在高温下生长，但对喜树碱敏感。这表明Bdf 2在高温下是有缺陷的。在半容许温度下，pot 1 bdf 2-t突变体的细胞周期被延迟在G2和/或M期。此外，一个温度敏感突变体的mst 1，编码组蛋白乙酰转移酶，显示了一个合成的生长缺陷与apot 1破坏在半容许温度。我们的研究结果表明，Bdf 2和Mst 1所需的细胞生长的圆形染色体。
Circular chromosomes have frequently been observed in tumors of mesenchymal origin. In the fission yeastSchizosaccharomyces pombe, deletion ofpot1+results in rapid telomere loss, and the resulting survivors have circular chromosomes. Fission yeast has 2 bromodomain and extra-terminal (BET) proteins, Bdf1 and Bdf2; both are required for maintaining acetylated histones. Here, we found thatbdf2, but notbdf1, was synthetically lethal withpot1. We also obtained a temperature-sensitivebdf2-tsmutant, which can grow at high temperatures but becomes camptothecin sensitive. This suggests that Bdf2 is defective at high temperatures. The cell cycle ofthe pot1 bdf2-tsmutant was delayed in the G2 and/or M phase at a semipermissive temperature. Furthermore, a temperature-sensitive mutant ofmst1, which encodes histone acetyltransferase, showed a synthetic growth defect with apot1disruptant at a semipermissive temperature. Our results suggest that Bdf2 and Mst1 are required for the growth of cells with circular chromosomes.