Immunogenicity of a multicomponent DNA vaccine against visceral leishmaniasis in dogs.
Immunogenicity of a multicomponent DNA vaccine against visceral leishmaniasis in dogs.
复制标题
多组分 DNA 疫苗针对犬内脏利什曼病的免疫原性。
DOI：
10.1016/j.vaccine.2005.10.052
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Melby,PeterC
中科院分区：
文献类型：
--
作者：
Saldarriaga,OmarA;Travi,BrunoL;Park,Won;Perez,LuisE;Melby,PeterC
Vaccination of dogs, the domestic reservoir of Leishmania chagasi, could not only decrease the burden of canine visceral leishmaniasis (VL), but could also indirectly reduce the incidence of human VL. Intramuscular vaccination of foxhounds with a Leishmania multicomponent (10 antigen) DNA vaccine resulted in antigen-induced lymphoproliferative and IFN-γ (but not IL-4) responses. This response was not augmented by co-administration of canine IL-12 or GM-CSF DNA adjuvants. The multicomponent DNA vaccine also induced a delayed type hypersensitivity (DTH) response to viable L. donovani promastigotes and led to a reduction of parasite burden in an in vitro intracellular infection model, and in the draining lymph node of dogs early after cutaneous challenge. Thus, the multicomponent DNA vaccine was effective in priming dogs for a parasite-specific type 1 cellular immune response, which was able to restrict parasite growth.
