Diel light:dark cycles significantly reduce FFA accumulation in FFA producing mutants of Synechocystis sp PCC 6803 compared to continuous light
Diel light:dark cycles significantly reduce FFA accumulation in FFA producing mutants of Synechocystis sp PCC 6803 compared to continuous light
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DOI：
10.1016/j.algal.2015.10.014
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发表时间：
2015-11-01
影响因子：
5.1
通讯作者：
Peebles, Christie A. M.
中科院分区：
文献类型：
--
作者：
Cheah, Yi Ern;Zimont, Allison J.;Peebles, Christie A. M.
我们研究了昼夜光暗循环（12：12 LD）对集胞藻PCC 6803中游离脂肪酸（FFA）产生的影响。我们的研究结果表明，FFA生产菌株的集胞藻FFA滴度显着降低，在12：12 LD相比，连续光。在转录水平上，我们没有看到脂肪酸生物合成途径的显着变化，作为FFA生产增加的结果。此外，我们描述了我们的努力，通过引入'tesA从大肠杆菌增加FFA生产。尽管有证据表明tesA mRNA转录本丰度，但在我们的集胞藻突变体中没有观察到可测量的TesA酶活性。总的来说，我们的工作表明了在蓝藻的代谢工程中考虑昼夜光暗周期的影响的重要性。(C)2015 Elsevier B.V.版权所有。
We examine the effect of diel light: dark cycles (12: 12 LD) on the production of free fatty acids (FFAs) in Synechocystis sp. PCC 6803. Our results show that FFA titers in FFA producing strains of Synechocystis were significantly reduced in 12: 12 LD compared to continuous light. On the transcriptional level, we saw no significant changes of the fatty acid biosynthesis pathway as a result of increased FFA production. In addition, we describe our efforts in increasing FFA production by introducing 'tesA from Escherichia coli. Despite evidence of 'tesA mRNA transcript abundance, no measurable TesA enzymatic activity was observed in our Synechocystis mutants. Overall, our work demonstrates the importance of considering the effects of diel light: dark cycles in the metabolic engineering of cyanobacteria. (C) 2015 Elsevier B.V. All rights reserved.