Roles of erbB4, rhombomere-specific, and rhombomere-independent cues in maintaining neural crest-free zones in the embryonic head
Roles of erbB4, rhombomere-specific, and rhombomere-independent cues in maintaining neural crest-free zones in the embryonic head
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DOI：
10.1016/j.ydbio.2003.11.003
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发表时间：
2004-02-15
影响因子：
2.7
通讯作者：
Gassmann, M
中科院分区：
文献类型：
--
作者：
Golding, JP;Sobieszczuk, D;Gassmann, M
在发育中的脊椎动物头部内，神经管衍生的神经嵴细胞（NCC）通过颅间充质的迁移被图案化为三个流，其中邻近菱形体（r）3和r5的间充质保持无NCC。受体酪氨酸激酶erbB 4在r3和r5内表达，并且是维持小鼠胚胎中r3邻近的无NCC区所必需的。在这项研究中，我们证明，r3参与模式小鼠NCC迁移的程度仅限于相同的背外侧区域erbB 4调节。在鸡胚中，我们发现，erb 134信号同样保持r3相邻的NCC-自由区。然而，虽然r5表达erb 134，这是不够的，以保持r3相邻的NCC-无区在移植实验中，r5取代r3，表明erbB 4需要额外的因素在A-P水平的r3模式NCC迁移。此外，我们表明，R5相邻的NCC-自由区是独立于R5，但需要表面外胚层。最后，我们证明，鸟类颅面外胚层的分子模式，与背外侧表面外胚层在r2/3和C的水平表达硫酸酯酶QSulf 1鹌鹑，或直向同源物CSulf 1在鸡。异常NCC迁移到r3相邻间充质与更集中的QSulf 1表达在r2/3表面外胚层。(C)2003年爱思唯尔公司All rights reserved.
Within the developing vertebrate head, the migration of neural tube-derived neural crest cells (NCCs) through the cranial mesenchyme is patterned into three streams, with mesenchyme adjacent to rhombomeres (r)3 and r5 maintained NCC-free. The receptor tyrosine kinase erbB4 is expressed within r3 and r5 and is required to maintain the r3-adjacent NCC-free zone in mouse embryos. In this study, we demonstrate that the extent of r3 involvement in patterning mouse NCC migration is restricted to the same dorsolateral region regulated by erbB4. In chick embryos, we show that erb134 signaling similarly maintains the r3-adjacent NCC-free zone. However, although r5 expresses erb134, this is insufficient to maintain the r3-adjacent NCC-free zone in grafting experiments where r5 replaced r3, indicating that erbB4 requires additional factors at the A-P level of r3 to pattern NCC migration. Furthermore, we show that the r5-adjacent NCC-free zone is maintained independently of r5, but requires surface ectoderm. Finally, we demonstrate that avian cranial surface ectoderm is patterned molecularly, with dorsolateral surface ectoderm at the levels of r2/3 and C expressing the sulfatase QSulf1 in quail, or the orthologue CSulf1 in chick. Aberrant NCC migration into r3-adjacent mesenchyme correlated with more focused QSulf1 expression in r2/3 surface ectoderm. (C) 2003 Elsevier Inc. All rights reserved.