Rapid volumetric imaging with Bessel-Beam three-photon microscopy
Rapid volumetric imaging with Bessel-Beam three-photon microscopy
复制标题
使用贝塞尔光束三光子显微镜进行快速体积成像
DOI：
10.1364/boe.9.001992
复制
发表时间：
2018-04-01
影响因子：
3.4
通讯作者：
Wang, Aimin
中科院分区：
文献类型：
--
作者：
Chen, Bingying;Huang, Xiaoshuai;Wang, Aimin
Owing to its tissue-penetration ability, multi-photon fluorescence microscopy allows for the high-resolution, non-invasive imaging of deep tissue in vivo; the recently developed three-photon microscopy (3PM) has extended the depth of high-resolution, noninvasive functional imaging of mouse brains to beyond 1.0 mm. However, the low repetition rate of femtosecond lasers that are normally used in 3PM limits the temporal resolution of point-scanning three-photon microscopy. To increase the volumetric imaging speed of 3PM, we propose a combination of an axially elongated needle-like Bessel-beam with three-photon excitation (3PE) to image biological samples with an extended depth of focus. We demonstrate the higher signal-to-background ratio (SBR) of the Bessel-beam 3PM compared to the two-photon version both theoretically and experimentally. Finally, we perform simultaneous calcium imaging of brain regions at different axial locations in live fruit flies and rapid volumetric imaging of neuronal structures in live mouse brains. These results highlight the unique advantage of conducting rapid volumetric imaging with a high SBR in the deep brain in vivo using scanning Bessel-3PM. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
