Hydrodynamic properties of a thromboxane A2/prostaglandin H2 antagonist binding site solubilized from human platelets.
Hydrodynamic properties of a thromboxane A2/prostaglandin H2 antagonist binding site solubilized from human platelets.
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从人血小板中溶解的血栓素 A2/前列腺素 H2 拮抗剂结合位点的流体动力学特性。
DOI：
10.1016/0006-291x(85)91901-1
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发表时间：
1985
影响因子：
3.1
通讯作者：
Halushka,PV
中科院分区：
文献类型：
--
作者：
Burch,RM;Mais,DE;Pepkowitz,SH;Halushka,PV
研究了血栓素A2/前列腺素H2受体拮抗剂9，11-二甲基亚甲基-11，12-亚甲基-16-（3-碘-4-羟基苯基）-13，14-二氢-13-氮杂-15 αβ-ω-四去甲-血栓烷A2（I-PTA-OH）在来自人血小板的溶解的膜蛋白中使用洗涤剂3-[（3-胆酰胺丙基）-二甲基铵基] 1-丙烷-磺酸盐（CHAPS）。凝胶过滤显示Stokes半径为5.25±0.37 nm（n= 9）。假设球形蛋白质，通过凝胶过滤测定的分子量为180，000 - 220，000道尔顿。通过蔗糖或甘油梯度的沉降显示沉降系数为6.3±0.2 Svedberg单位（n= 5）。使用斯托克斯半径和沉降系数计算的分子量为140，000道尔顿。摩擦比f f o为1.4，对应于7：1的轴向比。
The hydrodynamic properties of a binding site for the thromboxane A 2/prostaglandin H 2 receptor antagonist 9, 11-dimethylmethano-11, 12-methano-16-(3-iodo-4-hydroxyphenyl)-13, 14-dihydro-13-aza-15αβ-ω-tetranor-thromboxane A 2 (I-PTA-OH) were determined in solubilized membrane proteins from human platelets using the detergent 3-[(3-cholamidopropyl)-dimethylammonio] 1-propane-sulfonate (CHAPS). Gel filtration revealed a Stokes radius of 5.25±0.37 nm (n= 9). Molecular weight determined by gel filtration assuming a spherical protein was 180,000–220,000 Daltons. Sedimentation through sucrose or glycerol gradients revealed a sedimentation coefficient of 6.3±0.2 Svedberg units (n= 5). The molecular weight calculated using the Stokes radius and sedimentation coefficient was 140,000 Daltons. The frictional ratio f f o was 1.4, corresponding to an axial ratio of 7: 1.