Depression of serotonin clearance by rat lungs during oxygen exposure.
Depression of serotonin clearance by rat lungs during oxygen exposure.
复制标题
暴露于氧气期间大鼠肺部血清素清除率下降。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
A. Fisher
中科院分区：
文献类型：
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作者：
E. Block;A. Fisher
在正常或维生素E缺乏的Sprague-Dawley大鼠中评价氧（O2）暴露对离体灌流大鼠肺从灌流液中清除5-羟色胺（5-HT）的能力的影响。将大鼠暴露于100%O2（1 ATA）中4-48 h。随后分离肺，人工通气，并在再循环系统中用含有3%牛血清白蛋白和0.25 μ M [14 C] 5-HT的Krebs-Ringer碳酸氢盐溶液（pH 7.4）灌注。根据[14 C] 5-HT从灌注液中的消失率计算5-HT清除率。在暴露于100%O2的正常大鼠中，随着O2暴露时间的增加，5-HT的清除率逐渐降低。与空气暴露对照组相比，18 h后清除率降低20%（P <0.01），24 h后降低22%（P <0.01），48 h后降低35%（P <0.001）。与维生素E缺乏的大鼠，在5-HT清除率的减少发生在较短的曝光时间后，是一个更大的幅度比正常饮食的大鼠。肺对5-HT清除的抑制是由于高氧引起的肺功能的早期改变，维生素E缺乏可增强这种改变。5-HT清除率降低的最可能机制是干扰肺内皮细胞的转运特性。
The effect of oxygen (O2) exposure on the ability of the isolated, perfused rat lung to clear serotonin (5-hydroxytryptamine, 5-HT) from the perfusate was evaluated in normal or vitamin E-deficient Sprague-Dawley rats. Rats were exposed to 100% O2 at 1 ATA for 4-48 h. Lungs were subsequently isolated, artificially ventilated, and perfused in a recirculating system with Krebs-Ringer bicarbonate solution, pH 7.4 containing 3% bovine serum albumin and 0.25 muM [14C] 5-HT. 5HT clearance was calculated from the disappearance rate of [ 14C] 5-HT from the perfusate. In normal rats exposed to 100% O2, there was a progressive reduction in the clearance of 5-HT with increasing duration of O2 exposure. Compared to lungs from air-exposed controls, clearance was depressed 20% (P less than 0.01) after 18 h, 22% (P less than 0.01) after 24 h, and 35% (P less than 0.001) after 48 h. With vitamin E-deficient rats, the reduction in 5-HT clearance occurred after a shorter exposure time and was of greater magnitude than in rats on a normal diet. Depression of 5HT clearance by the lungs is an early alteration of lung function fue to hyperoxia and is potentiated by vitamin E deficiency. The most likely mechanism for the depression of 5-HT clearance is interference with the transport properties of lung endothelium.