USE OF SALICYLATE WITH HIGH-PRESSURE LIQUID CHROMATOGRAPHY AND ELECTROCHEMICAL DETECTION AS A SENSITIVE MEASURE OF HYDROXYL FREE RADICALS IN ADRIAMYCIN TREATED RATS
USE OF SALICYLATE WITH HIGH-PRESSURE LIQUID CHROMATOGRAPHY AND ELECTROCHEMICAL DETECTION AS A SENSITIVE MEASURE OF HYDROXYL FREE RADICALS IN ADRIAMYCIN TREATED RATS
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DOI：
10.1016/0748-5514(86)90118-2
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
WONG P K
中科院分区：
文献类型：
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作者：
FLOYD R A;HENDERSON R;WONG P K
羟基自由基与水杨酸盐反应生成2，3-二羟基苯甲酸(2，3-DHBA)和2，5-二羟基苯甲酸(2，5-DHBA)。利用高压液相色谱-电化学检测(LCED)技术，可以在分子水平上检测DHBA。由于水杨酸盐相对无毒，我们将其作为捕捉剂使用，首次尝试检查LCED方法作为体内.ovrhdot.OH产生的灵敏测量方法的使用。以阿霉素诱导氧自由基组织损伤为模型，我们发现，与对照组相比，药物处理组大鼠心脏和肌肉中DHBA的水平分别增加了100倍，肺中增加了30倍，脑和血液中增加了30倍。这些首次的观察结果支持了阿霉素在组织中诱导.ovrhdot.OH的理论，并表明LCED方法可能被证明是测量体内氧自由基产生的有用方法。
Hydroxyl free radicals react with salicylate to form 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA). Utilizing the technique of high pressure liquid chromatography with electrochemical detection (LCED), it is possible to detect DHBAs at the level of femtomoles. Since salicylate is relatively non-toxic, we have administered it as a trapping agent in a first attempt to examine the use of the LCED method as a sensitive measure of in vivo .ovrhdot.OH production. Utilizing adriamycin administration as a model to induce oxygen free radical tissue damage, we found that the level of DHBAs present in drug treated rats versus controls was increased 100-fold in heart and muscle, 30-fold in lung, and 30-fold in brain and blood, respectively. These first observations support the theory that adriamycin induces .ovrhdot.OH in tissue and indicate that the LCED method may prove to be useful to measure oxygen free radical production in vivo.